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Literature summary extracted from

  • Klement, H.; Helfrich, M.; Oster, U.; Schoch, S.; Rudiger, W.
    Pigment-free NADPH:protochlorophyllide oxidoreductase from Avena sativa L: purification and substrate specificity (1999), Eur. J. Biochem., 265, 862-874.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.33
-
 Hordeum vulgare
1.3.1.33
-
 Lathyrus oleraceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.33 etioplast
-
 Arabidopsis thaliana 9513
-
1.3.1.33 etioplast
-
 Avena sativa 9513
-
1.3.1.33 etioplast
-
 Hordeum vulgare 9513
-
1.3.1.33 etioplast
-
 Lathyrus oleraceus 9513
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.33 Mg2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Avena sativa
1.3.1.33 Mg2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Hordeum vulgare
1.3.1.33 Zn2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Avena sativa
1.3.1.33 Zn2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.33 34000
-
 2 related peptides of 34000/36000 Avena sativa
1.3.1.33 36000
-
 2 related peptides of 36000/38000 Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH Hordeum vulgare
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Lathyrus oleraceus
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Arabidopsis thaliana
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Avena sativa key enzyme for light-dependent chlorophyll biosynthesis chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Arabidopsis thaliana
-
-
-
1.3.1.33 Avena sativa
-
 L.cv. Pirol, var. Irlbad
-
1.3.1.33 Hordeum vulgare
-
 barley
-
1.3.1.33 Lathyrus oleraceus
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.33 seedling
-
 Arabidopsis thaliana
-
1.3.1.33 seedling
-
 Avena sativa
-
1.3.1.33 seedling
-
 Hordeum vulgare
-
1.3.1.33 seedling
-
 Lathyrus oleraceus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.33 additional information
-
-
 Hordeum vulgare
1.3.1.33 additional information
-
 1.49 chlorophyllide synthesis/flash per mg protein Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH
-
 Hordeum vulgare chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Lathyrus oleraceus chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Arabidopsis thaliana chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Avena sativa chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH key enzyme for light-dependent chlorophyll biosynthesis Avena sativa chlorophyllide + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 POR A
-
 Arabidopsis thaliana
1.3.1.33 POR A
-
 Hordeum vulgare
1.3.1.33 POR B
-
 Arabidopsis thaliana
1.3.1.33 POR B
-
 Hordeum vulgare