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Literature summary extracted from

  • Griffiths, W.T.
    Substrate-specificity studies on protochlorophyllide reductase in barley (Hordeum vulgare) etioplast membranes (1980), Biochem. J., 186, 267-278.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.33 etioplast
-
 Hordeum vulgare 9513
-
1.3.1.33 etioplast prolamellar body
-
 Hordeum vulgare 9541
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.33 Mg2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Hordeum vulgare
1.3.1.33 Zn2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 additional information Hordeum vulgare naturally occurring esterified protochlorophyllide and chemically prepared protochlorophyllide methyl ester are not substrates for the enzyme, removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate ?
-
 ?
1.3.1.33 protochlorophyllide + NADPH Hordeum vulgare biosynthetic pathway leading to chlorophyll a chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Hordeum vulgare
-
 barley
-
1.3.1.33 Hordeum vulgare
-
 L., cultivar Proctor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 additional information naturally occurring esterified protochlorophyllide and chemically prepared protochlorophyllide methyl ester are not substrates for the enzyme, removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate Hordeum vulgare ?
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Hordeum vulgare chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH biosynthetic pathway leading to chlorophyll a Hordeum vulgare chlorophyllide + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH no reaction with NADH Hordeum vulgare