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Literature summary extracted from
- The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Isolation and characterization of the NADPH: protochlorophyllide oxidoreductase (1980), Eur. J. Biochem., 111, 251-258.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.1.33 | active enzyme is protected by NADPH, protochlorophyllide and glycerol | Hordeum vulgare |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.1.33 | etioplast | - |
Hordeum vulgare | 9513 | - |
| 1.3.1.33 | etioplast prolamellar body | - |
Hordeum vulgare | 9541 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.33 | 36000 | - |
SDS-PAGE | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.33 | protochlorophyllide + NADPH | Hordeum vulgare | - |
chlorophyllide + NADP+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.33 | Hordeum vulgare | - |
barley | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.33 | - |
Hordeum vulgare |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.1.33 | leaf | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.33 | additional information | - |
1.6 nmol chlorophyllide/mg protein/flash | Hordeum vulgare |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.1.33 | 0°C, can be kept in elution buffer for at least 3h without any detectable loss of activity | Hordeum vulgare |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.33 | protochlorophyllide + NADPH | - |
Hordeum vulgare | chlorophyllide + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.1.33 | monomer | 1 * 36000, SDS-PAGE | Hordeum vulgare |
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Release 2023.1 (January 2023)
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