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Literature summary extracted from

  • Haas, M.; Beyer, D.; Gahlmann, R.; Freiberg, C.
    YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases (2001), Microbiology, 147, 1783-1791.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.88 medicine attractive target for antibacterial drug discovery Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 Def and YkrB, expression in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 actinonin 50% inhibition of enzyme Def at 95 nM, 50% inhibition of enzyme form YkrB by 130 nM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.88 2.3
-
N-formyl-Met-Ala-Ser enzyme YkrB Bacillus subtilis
3.5.1.88 3
-
N-formyl-Met-Ala-Ser enzyme Def Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Bacillus subtilis
-
harbours two functional peptide deformylase: Def and YkrB. YkrB probably is the predominant deformylase
-
3.5.1.88 Pseudomonas aeruginosa
-
two functional deformylases
-
3.5.1.88 Streptococcus pneumoniae
-
two functional deformylases
-
3.5.1.88 Streptococcus pyogenes
-
two functional deformylases
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 Def and YkrB, recombinant enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 N-formyl-Met-Ala-Ser + H2O
-
Bacillus subtilis formate + Met-Ala-Ser
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.88 0.07
-
N-formyl-Met-Ala-Ser enzyme Def Bacillus subtilis
3.5.1.88 0.17
-
N-formyl-Met-Ala-Ser enzyme YkrB Bacillus subtilis