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Literature summary extracted from

  • Kruus, K.; Andreacchi, A.; Wang, W.K.; Wu, J.H.D.
    Product inhibition of the recombinant CelS, and exoglucanase component of the Clostridium thermocellum cellulosome (1995), Appl. Microbiol. Biotechnol., 44, 399-404.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 cloned in Escherichia coli Acetivibrio thermocellus
3.2.1.176 expression in Escherichia coli Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 arbutin 20 mg/ml, 30% inhibition Acetivibrio thermocellus
3.2.1.176 cellobiose inhibits recombinant CelS activity on cellopentaose. Complete inhibition at 10 mg/ml cellobiose. Cellobiose appeared to inhibit the CelS activity through a competitive mechanism. The inhibition is relieved when beta-glucosidase is added, presumably because of the conversion of cellobiose into glucose Acetivibrio thermocellus
3.2.1.176 lactose inhibits recombinant CelS activity on cellopentaose, 81% inhibition at 20 mg/ml lactose Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 1.4
-
 cellopentaose pH 5.7, temperature not specified in the publication Acetivibrio thermocellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Acetivibrio thermocellus
-
-
-
3.2.1.176 Acetivibrio thermocellus A3DH67
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176
-
 Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 avicel + H2O
-
 Acetivibrio thermocellus cellobiose
-
 ?
3.2.1.176 cellopentaose + H2O
-
 Acetivibrio thermocellus cellobiose + cellotriose
-
 ?
3.2.1.176 cellulose + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Acetivibrio thermocellus cellobiose + D-glucose + cellotriose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 celS
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 60
-
-
 Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 5.7
-
 assay at Acetivibrio thermocellus

General Information

EC Number General Information Comment Organism
3.2.1.176 physiological function CelS is the exoglucanase component of the Clostridium thermocellum cellulosome multicomponent cellulase complex Acetivibrio thermocellus