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Literature summary extracted from

  • Fracheboud, D.; Canevascini, G.
    Isolation, purification, and properties of the exocellulase from Sporotrichum (Chrysosporium) thermophile (1989), Enzyme Microb. Technol., 11, 220-229.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 Ag+ slight Thermothelomyces heterothallicus
3.2.1.91 Ag+ 1 mM, slight inhibition Thermothelomyces thermophilus
3.2.1.91 cellobiose
-
 Thermothelomyces heterothallicus
3.2.1.91 cellobiose
-
 Thermothelomyces thermophilus
3.2.1.91 Cu2+ slight Thermothelomyces heterothallicus
3.2.1.91 Cu2+ 1 mM, slight inhibition Thermothelomyces thermophilus
3.2.1.91 Fe3+
-
 Thermothelomyces heterothallicus
3.2.1.91 Fe3+ 0.5 mM, 50% inhibition Thermothelomyces thermophilus
3.2.1.91 Hg2+
-
 Thermothelomyces heterothallicus
3.2.1.91 Hg2+ 1 mM, extremely inhibitory Thermothelomyces thermophilus
3.2.1.91 lactose
-
 Thermothelomyces heterothallicus
3.2.1.91 lactose
-
 Thermothelomyces thermophilus
3.2.1.91 additional information no inhibition at 1 mM: EDTA, citrate, o-phenanthroline, 8-hydroxyquinoline. Iodoacetate, N-ethylmaleimide, and 4-hydroxymercuribenzoate hardly affected the reaction at up to 1 mM final inhibitor concentration. Glucose has no effect up to 10 mM Thermothelomyces thermophilus
3.2.1.91 N-bromosuccinimide
-
 Thermothelomyces heterothallicus
3.2.1.91 N-bromsuccinimide
-
 Thermothelomyces thermophilus
3.2.1.91 Pb2+ slight Thermothelomyces heterothallicus
3.2.1.91 Pb2+ 1 mM, slight inhibition Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 3
-
 p-Nitrophenyl-lactoside
-
 Thermothelomyces heterothallicus
3.2.1.91 3
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 30°C Thermothelomyces thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.91 extracellular
-
 Thermothelomyces heterothallicus
-
-
3.2.1.91 extracellular
-
 Thermothelomyces thermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.91 63750
-
 SDS-PAGE Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Thermothelomyces heterothallicus
-
-
-
3.2.1.91 Thermothelomyces thermophilus
-
-
-
3.2.1.91 Thermothelomyces thermophilus ATCC 42464
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.91 glycoprotein 8% reducing sugar Thermothelomyces heterothallicus
3.2.1.91 glycoprotein contains 5.4% mannose and 1.7% glucose with traces of galactose and glucosamine Thermothelomyces thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91
-
 Thermothelomyces heterothallicus
3.2.1.91
-
 Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.91 0.32
-
-
 Thermothelomyces heterothallicus
3.2.1.91 0.32
-
 pH 5.0, 30°C, substrate: 4-nitrophenyl beta-D-lactoside Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-lactoside + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + lactose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-lactoside + H2O
-
 Thermothelomyces thermophilus ATCC 42464 4-nitrophenol + lactose
-
 ?
3.2.1.91 cellodextrins + H2O
-
 Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose
-
 ?
3.2.1.91 cellotetraose + H2O
-
 Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose different combinations and proportions of products ?
3.2.1.91 cellotriitol + H2O
-
 Thermothelomyces heterothallicus cellobiose + sorbitol
-
 ?
3.2.1.91 cellotriose + H2O
-
 Thermothelomyces heterothallicus cellobiose + glucose
-
 ?
3.2.1.91 cellulose + H2O
-
 Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ?
3.2.1.91 cellulose + H2O hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose Thermothelomyces thermophilus cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose Thermothelomyces thermophilus ATCC 42464 cellobiose + ?
-
 ?
3.2.1.91 additional information not: cellobiose Thermothelomyces heterothallicus ?
-
 ?
3.2.1.91 p-nitrophenyl-lactoside + H2O
-
 Thermothelomyces heterothallicus p-nitrophenol + lactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 63750, SDS-PAGE Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 40
-
 3 hours Thermothelomyces heterothallicus
3.2.1.91 80
-
 50% loss of activity after 3 hours Thermothelomyces heterothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 3.5
-
 substrate: amorphous cellulose Thermothelomyces thermophilus
3.2.1.91 3.5 4 amorphous cellulose Thermothelomyces heterothallicus
3.2.1.91 4 5 crystalline cellulose Thermothelomyces heterothallicus
3.2.1.91 5
-
 p-nitrophenyl-lactoside Thermothelomyces heterothallicus
3.2.1.91 5
-
 substrate: 4-nitrophenyl beta-D-lactoside Thermothelomyces thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.91 0.7
-
 cellobiose pH 5.0, 30°C Thermothelomyces thermophilus
3.2.1.91 1.5
-
 lactose pH 5.0, 30°C Thermothelomyces thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.91 Thermothelomyces thermophilus isoelectric focusing
-
 4.52