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Literature summary extracted from
- Partial purification and properties of a beta-N-acetylglucosaminidase from the fungus Sclerotina fructigena (1973), Biochem. J., 131, 381-388.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.52 | N-acetyl-beta-D-glucosaminolactone | - |
Sclerotinia fructigena |  |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | inhibition by excess of substrate | Sclerotinia fructigena | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.52 | 0.52 | - |
N,N-diacetylchitobiose | - |
Sclerotinia fructigena | |
| 3.2.1.52 | 2 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Sclerotinia fructigena | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.52 | extracellular | - |
Sclerotinia fructigena | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 141000 | - |
gel filtration | Sclerotinia fructigena |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Sclerotinia fructigena | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | Sclerotinia fructigena | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.52 | 46.7 fold to homogeneity | Sclerotinia fructigena |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | culture filtrate | - |
Sclerotinia fructigena | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | additional information | - |
- |
Sclerotinia fructigena |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.52 | 0°C, half-life of the purified enzyme is 6 days | Sclerotinia fructigena |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Sclerotinia fructigena | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside | - |
? | |
| 3.2.1.52 | 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Sclerotinia fructigena | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
| 3.2.1.52 | chitin + H2O | after 90 h at 37°C and pH 5.0, poor substrate | Sclerotinia fructigena | N-acetylglucosamine | - |
? | |
| 3.2.1.52 | N,N'-diacetylchitobiose + H2O | - |
Sclerotinia fructigena | N-acetyl-D-glucosamine | - |
? | |
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Sclerotinia fructigena | N-acetyl-D-hexosamines | - |
? | |
| 3.2.1.52 | p-nitrophenyl galactosaminide + H2O | - |
Sclerotinia fructigena | p-nitrophenol + galactosamine | - |
? | |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Sclerotinia fructigena | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| 3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Sclerotinia fructigena | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 37 | - |
crude enzyme, relatively stable | Sclerotinia fructigena |
| 3.2.1.52 | 50 | - |
50% inactivation after 10 min | Sclerotinia fructigena |
| 3.2.1.52 | 60 | - |
complete inactivation after 10 min | Sclerotinia fructigena |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 4.4 | - |
N-acetylgalactosaminidase activity | Sclerotinia fructigena |
| 3.2.1.52 | 5.4 | - |
N-acetylglucosaminidase activity | Sclerotinia fructigena |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 2 | - |
complete inactivation after 30 min at 25°C | Sclerotinia fructigena |
| 3.2.1.52 | 3 | - |
40% inactivation after 30 min at 25°C | Sclerotinia fructigena |
| 3.2.1.52 | 4 | 11 | stable | Sclerotinia fructigena |
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