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Literature summary extracted from

  • Ceccarini, C.; D'Aniello, A.; Cacace, M.G.; Atkinson, P.H.
    Purification and characterization of a beta-N-acetylglucosaminidase from Octopus vulgaris. Determination of specificity by using 360-MHz 1H-NMR spectroscopy (1983), Eur. J. Biochem., 132, 469-476.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.527
-
 phenyl-N-acetyl-beta-D-glucosaminide
-
 Octopus vulgaris
3.2.1.52 0.617
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Octopus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 27000
-
 2 * 27000 + 2 * 34000, SDS-PAGE Octopus vulgaris
3.2.1.52 34000
-
 2 * 27000 + 2 * 34000, SDS-PAGE Octopus vulgaris
3.2.1.52 120000
-
 gel filtration Octopus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Octopus vulgaris
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Octopus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 types I-IV 150fold, type I to homogeneity Octopus vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Octopus vulgaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 hepatopancreas
-
 Octopus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 62.9
-
 substrate: phenyl-N-acetyl-beta-D-glucosaminide Octopus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Octopus vulgaris N-acetyl-D-hexosamines
-
 ?
3.2.1.52 ovalbumin glycopeptides + H2O ovalbumin glycopeptide AC-A with the sugar composition: Gal,GlcNAc5,Man5,Asn Octopus vulgaris ?
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Octopus vulgaris 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Octopus vulgaris 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 phenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Octopus vulgaris phenol + N-acetyl-beta-D-glucosaminide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 tetramer 2 * 27000 + 2 * 34000, SDS-PAGE Octopus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 55
-
 forms II and IV Octopus vulgaris
3.2.1.52 55 65 form I Octopus vulgaris
3.2.1.52 60
-
 form III Octopus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3.5
-
 forms I-IV, substrate: phenyl-N-acetyl-beta-D-glucosaminide Octopus vulgaris