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Literature summary extracted from

  • Devery, R.; Collins, P.; Johnson, A.; Watson, G.
    Purification of pig gastric-mucosal N-acetyl-beta-D-glucosaminidase (1984), Biochem. Soc. Trans., 12, 460.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-acetyl-D-galactosamine PI: concentration of 30 mM causes 69% inhibition, PII: concentration of 30 mM causes 80% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.9
-
 4-Methylumbelliferyl beta-D-glucosaminide PI Sus scrofa
3.2.1.52 0.91
-
 4-Methylumbelliferyl beta-D-glucosaminide PII Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 lysosome
-
 Sus scrofa 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Sus scrofa
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 forms PI and PII, each 200fold Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 gastric mucosa
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl beta-D-glucosaminide + H2O
-
 Sus scrofa 4-methylumbelliferone + D-glucosamine
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Sus scrofa 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Sus scrofa N-acetyl-D-hexosamines
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 55
-
 PI: complete inactivation after 20 min, PII: after 1 h 30% of activity remain Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.7
-
 PI and PII Sus scrofa