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Literature summary extracted from

  • Cohen, R.J.
    beta-N-Acetylglucosaminidase from Phycomyces blakesleeanus (1986), Plant Sci., 43, 93-101.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N,N-diacetylchitobiose with 0.1 mM 81% and with 2 mM 32% of activity remains Phycomyces blakesleeanus
3.2.1.52 N-acetyl-D-glucosamine with 2 mM 85%, with 4 mM 75%, with 8 mM 65% and with 12 mM 59% of activity remains Phycomyces blakesleeanus
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Phycomyces blakesleeanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.3
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Phycomyces blakesleeanus
3.2.1.52 0.33
-
 N,N-diacetylchitobiose
-
 Phycomyces blakesleeanus
3.2.1.52 2.3
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Phycomyces blakesleeanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 72000
-
 gel filtration Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Phycomyces blakesleeanus
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Phycomyces blakesleeanus NRRL1555(-)
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Phycomyces blakesleeanus
-
 NRRL1555(-)
-
3.2.1.52 Phycomyces blakesleeanus NRRL1555(-)
-
 NRRL1555(-)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 partial, 5-10fold Phycomyces blakesleeanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 mycelium
-
 Phycomyces blakesleeanus
-
3.2.1.52 sporangiophore
-
 Phycomyces blakesleeanus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 4°C, stable for 6 weeks Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Phycomyces blakesleeanus N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Phycomyces blakesleeanus NRRL1555(-) N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Phycomyces blakesleeanus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Phycomyces blakesleeanus NRRL1555(-) N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Phycomyces blakesleeanus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Phycomyces blakesleeanus NRRL1555(-) 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Phycomyces blakesleeanus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Phycomyces blakesleeanus NRRL1555(-) 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 60
-
 complete loss of activity after 40 min, biphasic denaturation curve Phycomyces blakesleeanus
3.2.1.52 70
-
 95% of activity are lost after 2 min Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5
-
-
 Phycomyces blakesleeanus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 6.2
-
 80% of activity Phycomyces blakesleeanus