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Literature summary extracted from

  • Iwamoto, T.; Okiura, T.; Sasaki, T.; Inaoka, M.
    Kinds of glycosidase and properties of beta-N-acetylglucosaminidase bound to mycelia of Streptomyces sp. (1987), J. Ferment. Technol., 65, 593-596.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 galactosamine with 10 mM 84.0% of activity remains Streptomyces sp.
3.2.1.52 glucosamine with 10 mM 29.3% of activity remains Streptomyces sp.
3.2.1.52 N-acetyl-beta-D-glucosaminolactone with 1 mM 4.7% of activity and with 0.1 mM 6.6% of activity remains Streptomyces sp.
3.2.1.52 N-acetyl-D-galactosamine with 10 mM 52.6% of activity remains Streptomyces sp.
3.2.1.52 N-acetyl-D-glucosamine with 10 mM 64.2% of activity remains Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.25
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Streptomyces sp.
-
N-acetyl-D-hexosamines
-
?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Streptomyces sp. L-13
-
N-acetyl-D-hexosamines
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Streptomyces sp.
-
-
-
3.2.1.52 Streptomyces sp. L-13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 to homogeneity Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 mycelium
-
Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 12
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
Streptomyces sp. N-acetyl-D-hexosamines
-
?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
Streptomyces sp. L-13 N-acetyl-D-hexosamines
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Streptomyces sp. 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Streptomyces sp. L-13 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Streptomyces sp. 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Streptomyces sp. L-13 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6
-
-
Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4 8 stable at 30°C for 30 min Streptomyces sp.