BRENDA - Enzyme Database

Purification and characterization of beta-N-acetylhexosaminidase from the liver of a prawn, Penaeus japonicus

Koga, D.; Hoshika, H.; Matsushita, M.; Tanaka, A.; Ide, A.; Kono, M.; Biosci. Biotechnol. Biochem. 60, 194-199 (1996) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.52
Hg2+
concentration of 10 mM causes 39% inhibition
Penaeus japonicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.52
0.044
-
(N-acetyl-beta-D-glucosamine)4
-
Penaeus japonicus
3.2.1.52
0.045
-
(N-acetyl-beta-D-glucosamine)5
-
Penaeus japonicus
3.2.1.52
0.047
-
(N-acetyl-beta-D-glucosamine)6
-
Penaeus japonicus
3.2.1.52
0.055
-
(N-acetyl-beta-D-glucosamine)3
-
Penaeus japonicus
3.2.1.52
0.117
-
(N-acetyl-beta-D-glucosamine)2
-
Penaeus japonicus
3.2.1.52
0.137
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Penaeus japonicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.52
64000
-
SDS-PAGE
Penaeus japonicus
3.2.1.52
110000
-
gel filtration
Penaeus japonicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
Penaeus japonicus
-
N-acetyl-D-hexosamines
-
Penaeus japonicus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.52
Penaeus japonicus
-
prawn
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.52
699fold
Penaeus japonicus
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.2.1.52
hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
exo-type reaction
Penaeus japonicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.52
liver
-
Penaeus japonicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.52
123000
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Penaeus japonicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.52
(N-acetyl-beta-D-glucosamine)2 + H2O
-
393697
Penaeus japonicus
2 N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)3 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)4 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)5 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)6 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
393697
Penaeus japonicus
4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
-
?
3.2.1.52
chitin oligosaccharides + H2O
(N-acetyl-D-glucosamine)2-6
393697
Penaeus japonicus
N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
393697
Penaeus japonicus
?
-
-
-
?
3.2.1.52
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
393697
Penaeus japonicus
?
-
-
-
?
3.2.1.52
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
393697
Penaeus japonicus
2 N,N-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N',N''-triacetylchitotriose + H2O
-
393697
Penaeus japonicus
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N'-diacetylchitobiose + H2O
-
393697
Penaeus japonicus
2 N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
-
393697
Penaeus japonicus
N-acetyl-D-hexosamines
-
393697
Penaeus japonicus
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
393697
Penaeus japonicus
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
393697
Penaeus japonicus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.52
dimer
2 * 64000, SDS-PAGE
Penaeus japonicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.52
50
-
-
Penaeus japonicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.52
55
-
stable below
Penaeus japonicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.52
8.3
-
(N-acetyl-beta-D-glucosamine)6
-
Penaeus japonicus
3.2.1.52
14.7
-
(N-acetyl-beta-D-glucosamine)5
-
Penaeus japonicus
3.2.1.52
30.1
-
(N-acetyl-beta-D-glucosamine)4
-
Penaeus japonicus
3.2.1.52
96.4
-
(N-acetyl-beta-D-glucosamine)3
-
Penaeus japonicus
3.2.1.52
298
-
(N-acetyl-beta-D-glucosamine)2
-
Penaeus japonicus
3.2.1.52
598
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Penaeus japonicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.52
5
5.5
-
Penaeus japonicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.52
4
11
stable
Penaeus japonicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.52
Hg2+
concentration of 10 mM causes 39% inhibition
Penaeus japonicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.52
0.044
-
(N-acetyl-beta-D-glucosamine)4
-
Penaeus japonicus
3.2.1.52
0.045
-
(N-acetyl-beta-D-glucosamine)5
-
Penaeus japonicus
3.2.1.52
0.047
-
(N-acetyl-beta-D-glucosamine)6
-
Penaeus japonicus
3.2.1.52
0.055
-
(N-acetyl-beta-D-glucosamine)3
-
Penaeus japonicus
3.2.1.52
0.117
-
(N-acetyl-beta-D-glucosamine)2
-
Penaeus japonicus
3.2.1.52
0.137
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Penaeus japonicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.52
64000
-
SDS-PAGE
Penaeus japonicus
3.2.1.52
110000
-
gel filtration
Penaeus japonicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
Penaeus japonicus
-
N-acetyl-D-hexosamines
-
Penaeus japonicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.52
699fold
Penaeus japonicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.52
liver
-
Penaeus japonicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.52
123000
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Penaeus japonicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.52
(N-acetyl-beta-D-glucosamine)2 + H2O
-
393697
Penaeus japonicus
2 N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)3 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)4 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)5 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)6 + H2O
-
393697
Penaeus japonicus
(N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
393697
Penaeus japonicus
4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
-
-
?
3.2.1.52
chitin oligosaccharides + H2O
(N-acetyl-D-glucosamine)2-6
393697
Penaeus japonicus
N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
393697
Penaeus japonicus
?
-
-
-
?
3.2.1.52
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
393697
Penaeus japonicus
?
-
-
-
?
3.2.1.52
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
393697
Penaeus japonicus
2 N,N-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N',N''-triacetylchitotriose + H2O
-
393697
Penaeus japonicus
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N'-diacetylchitobiose + H2O
-
393697
Penaeus japonicus
2 N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
-
393697
Penaeus japonicus
N-acetyl-D-hexosamines
-
393697
Penaeus japonicus
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
393697
Penaeus japonicus
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
393697
Penaeus japonicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.52
dimer
2 * 64000, SDS-PAGE
Penaeus japonicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.52
50
-
-
Penaeus japonicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.52
55
-
stable below
Penaeus japonicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.52
8.3
-
(N-acetyl-beta-D-glucosamine)6
-
Penaeus japonicus
3.2.1.52
14.7
-
(N-acetyl-beta-D-glucosamine)5
-
Penaeus japonicus
3.2.1.52
30.1
-
(N-acetyl-beta-D-glucosamine)4
-
Penaeus japonicus
3.2.1.52
96.4
-
(N-acetyl-beta-D-glucosamine)3
-
Penaeus japonicus
3.2.1.52
298
-
(N-acetyl-beta-D-glucosamine)2
-
Penaeus japonicus
3.2.1.52
598
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Penaeus japonicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.52
5
5.5
-
Penaeus japonicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.52
4
11
stable
Penaeus japonicus