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Literature summary extracted from

  • Geimba, M.P.; Riffel, A.; Brandelli, A.
    Purification and characterization of beta-N-acetylhexosaminidase from the phytopathogenic fungus Bipolaris sorokiniana (1998), J. Appl. Microbiol., 85, 708-714.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 Mn2+
-
 Bipolaris sorokiniana
3.2.1.52 Ni2+
-
 Bipolaris sorokiniana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 acetate
-
 Bipolaris sorokiniana
3.2.1.52 Hg2+
-
 Bipolaris sorokiniana
3.2.1.52 N-acetyl-D-galactosamine with 60 mM 45% of activity remains Bipolaris sorokiniana
3.2.1.52 N-acetyl-D-glucosamine with 60 mM 24% of activity remains Bipolaris sorokiniana
3.2.1.52 N-acetylglucosamine-6-sulfate with 10 mM 86% of activity remains Bipolaris sorokiniana
3.2.1.52 N-acetylglucosamine-isothiocyanate with 10 mM 46% of activity remains Bipolaris sorokiniana
3.2.1.52 N-acetylmannosamine with 10 mM 83% of activity remains Bipolaris sorokiniana
3.2.1.52 Pb2+
-
 Bipolaris sorokiniana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.081
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bipolaris sorokiniana
3.2.1.52 1.24
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bipolaris sorokiniana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular
-
 Bipolaris sorokiniana
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 55000
-
 SDS-PAGE Bipolaris sorokiniana
3.2.1.52 120000
-
 gel filtration Bipolaris sorokiniana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bipolaris sorokiniana
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bipolaris sorokiniana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 70fold Bipolaris sorokiniana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 culture filtrate
-
 Bipolaris sorokiniana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 817.8
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bipolaris sorokiniana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bipolaris sorokiniana N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bipolaris sorokiniana 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bipolaris sorokiniana 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Bipolaris sorokiniana 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.52 p-nitrophenyl-chitobioside + H2O
-
 Bipolaris sorokiniana ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 55
-
-
 Bipolaris sorokiniana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 28
-
 stable Bipolaris sorokiniana
3.2.1.52 37
-
 25% inactivation after 30 min Bipolaris sorokiniana
3.2.1.52 55
-
 60% inactivation after 30 min, complete inactivation after 120 min Bipolaris sorokiniana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.5 5
-
 Bipolaris sorokiniana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4 10 stable, at 37°C Bipolaris sorokiniana