Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mark, B.L.; Wasney, G.A.; Salo, T.J.S.; Khan, A.M.; Cao, Z.; Robbins, P.W.; James, M.N.G.; Triggs-Raine, B.L.
    Structural and functional characterization of Streptomyces plicatus beta-N-acetylhexosaminidase by comparative molecular modeling and site-directed mutagenesis (1998), J. Biol. Chem., 273, 19618-19624.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 into Escherichia coli Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.52 D246N mutant Streptomyces sp.
3.2.1.52 E314Q mutant Streptomyces sp.
3.2.1.52 R162H mutant Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.02
-
 4-methylumbelliferyl beta-N-acetylglucosaminide E314Q Streptomyces sp.
3.2.1.52 0.14
-
 4-methylumbelliferyl beta-N-acetylglucosaminide wild type Streptomyces sp.
3.2.1.52 0.25
-
 4-methylumbelliferyl beta-N-acetylglucosaminide D246N Streptomyces sp.
3.2.1.52 5.67
-
 4-methylumbelliferyl beta-N-acetylglucosaminide R162H Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 55000
-
 recombinant SpHex, western blot analysis Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Streptomyces sp.
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Streptomyces sp.
-
 S. plicatus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 SpHex-MBP fusion protein Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 4°C, wild type and mutant enzymes, stable for at least 2 weeks Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl beta-N-acetylglucosaminide + H2O
-
 Streptomyces sp. 4-methylumbelliferone + beta-N-acetylglucosamine
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Streptomyces sp. N-acetyl-D-hexosamines
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 20
-
 stable for at least 48 h at room temperature, wild type and mutant enzymes Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3
-
 SpHex wild type Streptomyces sp.
3.2.1.52 3.8
-
 D246N Streptomyces sp.
3.2.1.52 6.5
-
 R162H Streptomyces sp.