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Literature summary extracted from

  • Del Pino, F.B.; Brandelli, A.; Termignoni, C.; Gonzales, J.C.; Henriques, J.A.P.; Dewes, H.
    Purification and characterization of beta-N-acetylhexosaminidase from bovine tick Boophilus microplus (Ixodide) larvae (1999), Comp. Biochem. Physiol. B, 123, 193-200.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 Mg2+
-
 Rhipicephalus microplus
3.2.1.52 Mn2+
-
 Rhipicephalus microplus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 antibodies inhibit about 90% of in vitro activity Rhipicephalus microplus
3.2.1.52 Hg2+ with 1 mM 13% of activity remains Rhipicephalus microplus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 microsome
-
 Rhipicephalus microplus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 60000
-
 SDS-PAGE Rhipicephalus microplus
3.2.1.52 127000
-
 gel filtration Rhipicephalus microplus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Rhipicephalus microplus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Rhipicephalus microplus
-
 bovine tick, ixodide
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein mannosylation Rhipicephalus microplus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 to homogeneity, 1564fold Rhipicephalus microplus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 hemolymph
-
 Rhipicephalus microplus
-
3.2.1.52 larva
-
 Rhipicephalus microplus
-
3.2.1.52 additional information no activity in saliva Rhipicephalus microplus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 77290
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Rhipicephalus microplus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Rhipicephalus microplus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Rhipicephalus microplus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Rhipicephalus microplus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 2 * 60000, SDS-PAGE Rhipicephalus microplus
3.2.1.52 dimer Hex A: alpha beta, Hex B: beta beta Rhipicephalus microplus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 65
-
-
 Rhipicephalus microplus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 60
-
 stable for 60 min at pH 4.7 Rhipicephalus microplus
3.2.1.52 80
-
 completely inactivated at 80°C and above Rhipicephalus microplus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.7
-
-
 Rhipicephalus microplus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4.5 7 at 4°C for 24 hours and at 37°C for 60 min Rhipicephalus microplus