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Literature summary extracted from

  • Bouquelet, S.; Spik, G.
    Characterization and localization of four forms of N-acetyl-beta-D-hexosaminidase from fenugreek (Trigonella foenum graecum) germinated seeds (1976), FEBS Lett., 63, 95-101.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-acetyl-beta-D-galactosaminolactone
-
 Trigonella foenum-graecum
3.2.1.52 N-acetyl-beta-D-glucosaminolactone
-
 Trigonella foenum-graecum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.04
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form I Trigonella foenum-graecum
3.2.1.52 0.04
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form IV Trigonella foenum-graecum
3.2.1.52 0.08
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form III Trigonella foenum-graecum
3.2.1.52 0.09
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form III Trigonella foenum-graecum
3.2.1.52 0.15
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form I Trigonella foenum-graecum
3.2.1.52 0.37
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form IV Trigonella foenum-graecum
3.2.1.52 0.55
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form II Trigonella foenum-graecum
3.2.1.52 0.62
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form II Trigonella foenum-graecum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Trigonella foenum-graecum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Trigonella foenum-graecum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 form I: 185fold, form II: 670fold, form III: 20880fold, form IV: 4740fold Trigonella foenum-graecum

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Trigonella foenum-graecum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 cotyledon forms I and II Trigonella foenum-graecum
-
3.2.1.52 embryo forms III and IV Trigonella foenum-graecum
-
3.2.1.52 endosperm form II Trigonella foenum-graecum
-
3.2.1.52 seed germinating seeds Trigonella foenum-graecum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 additional information
-
-
 Trigonella foenum-graecum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Trigonella foenum-graecum N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Trigonella foenum-graecum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Trigonella foenum-graecum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?