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Literature summary extracted from

  • Calvo, P.; Reglero, A.; Cabezas, J.A.
    Purification and properties of beta-N-acetylhexosaminidase from the mollusc Helicella ericetorum Muller (1978), Biochem. J., 175, 743-750.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 2-acetamido-2-deoxy-D-galactono(1-4)lactone complete inhibition with 100 mM Helicella ericetorum
3.2.1.52 D-mannose 75% inhibition with 100 mM Helicella ericetorum
3.2.1.52 Fe3+ concentration of 10 mM results in 85% inhibition Helicella ericetorum
3.2.1.52 Hg2+ concentration of 10 mM results in 97% inhibition Helicella ericetorum
3.2.1.52 N-acetyl-beta-D-glucosaminolactone complete inhibition with 100 mM Helicella ericetorum
3.2.1.52 N-acetyl-D-galactosamine 80% inhibition with 100 mM Helicella ericetorum
3.2.1.52 N-acetyl-D-glucosamine 80% inhibition with 100 mM Helicella ericetorum
3.2.1.52 Sodium acetate concentration of 10 mM results in 20% inhibition Helicella ericetorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.19
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Helicella ericetorum
3.2.1.52 0.43
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Helicella ericetorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 118000 147000 gel filtration Sephadex G-200 Helicella ericetorum
3.2.1.52 120000 145000 gel filtration Sephadex G-100 Helicella ericetorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Helicella ericetorum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Helicella ericetorum
-
 Müller
-
3.2.1.52 Helicella ericetorum Muller
-
 Müller
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 330fold Helicella ericetorum

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Helicella ericetorum
a
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose endo-type reaction Helicella ericetorum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 digestive gland
-
 Helicella ericetorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 7.8
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Helicella ericetorum
3.2.1.52 33
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Helicella ericetorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 chitin + H2O
-
 Helicella ericetorum N-acetylglucosamine
-
 ?
3.2.1.52 chitin + H2O
-
 Helicella ericetorum Muller N-acetylglucosamine
-
 ?
3.2.1.52 chondroitin 4-sulfate + H2O
-
 Helicella ericetorum N-acetylgalactosamine + ?
-
 ?
3.2.1.52 hyaluronic acid + H2O
-
 Helicella ericetorum N-acetylglucosamine + ?
-
 ?
3.2.1.52 hyaluronic acid + H2O
-
 Helicella ericetorum Muller N-acetylglucosamine + ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Helicella ericetorum N-acetyl-D-hexosamines
-
 ?
3.2.1.52 ovalbumin + H2O
-
 Helicella ericetorum N-acetylglucosamine + ?
-
 ?
3.2.1.52 ovalbumin + H2O
-
 Helicella ericetorum Muller N-acetylglucosamine + ?
-
 ?
3.2.1.52 ovomucoid + H2O
-
 Helicella ericetorum N-acetylglucosamine + ?
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Helicella ericetorum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Helicella ericetorum Muller 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Helicella ericetorum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Helicella ericetorum Muller 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 50
-
 stable for 100 min Helicella ericetorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside and -glucopyranoside Helicella ericetorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 3.3
-
 50% of activity are lost Helicella ericetorum
3.2.1.52 3.8 4.6 at 37°C stable for 2 hours Helicella ericetorum
3.2.1.52 6.3
-
 50% of activity are lost Helicella ericetorum