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Literature summary extracted from

  • Eriquez, L.A.; Pisano, M.A.
    Purification and characterization of an extracellular beta-N-acetylhexosaminidase from Paecilomyces persicinus (1979), J. Bacteriol., 137, 620-626.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.52 lyophilization results in marked decrease in activity Acremonium persicinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 beta-mercaptoethanol with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 22%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 20% Acremonium persicinum
3.2.1.52 Cd2+ with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 41%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 38% Acremonium persicinum
3.2.1.52 Hg2+ with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 24%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 20% Acremonium persicinum
3.2.1.52 N-acetyl-D-galactosamine with 10 mM the hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 26% Acremonium persicinum
3.2.1.52 N-acetyl-D-glucosamine with 10 mM the hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside is reduced to 32% Acremonium persicinum
3.2.1.52 Urea with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 37%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 34% Acremonium persicinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.322
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Acremonium persicinum
3.2.1.52 0.625
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Acremonium persicinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular
-
 Acremonium persicinum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 95000
-
 SDS-PAGE Acremonium persicinum
3.2.1.52 100000
-
 gel filtration Acremonium persicinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Acremonium persicinum
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Acremonium persicinum P-10
-
 N-acetyl-D-hexosamines
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.52 2-mercaptoethanol with 2 mM hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside is reduced to 22%, hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside to 20% Acremonium persicinum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Acremonium persicinum
-
 P-10
-
3.2.1.52 Acremonium persicinum P-10
-
 P-10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 68fold Acremonium persicinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 culture filtrate
-
 Acremonium persicinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 0.926
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Acremonium persicinum
3.2.1.52 5.64
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Acremonium persicinum

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, purified enzyme, stable Acremonium persicinum
3.2.1.52 refrigerated temperatures, stable for several days Acremonium persicinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Acremonium persicinum N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Acremonium persicinum P-10 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Acremonium persicinum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Acremonium persicinum P-10 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Acremonium persicinum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Acremonium persicinum P-10 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 35 37
-
 Acremonium persicinum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 10 70
-
 Acremonium persicinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 60
-
 temperatures above quickly inactivate the enzyme Acremonium persicinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.6
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Acremonium persicinum
3.2.1.52 5.8
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Acremonium persicinum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 2 10
-
 Acremonium persicinum