Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shigeta, S.; Matsuda, A.; Oka, S.
    Purification and characterization of a beta-N-acetylhexosaminidase of sea-squirt (1982), J. Biochem., 92, 163-172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ with 7 mM 5% of activity remains Styela plicata
3.2.1.52 Fe2+ with 7 mM 16% of activity remains Styela plicata
3.2.1.52 Hg2+ concentration of 7 mM results in total loss of activity Styela plicata
3.2.1.52 Monoiodoacetic acid with 1 mM 73% of activity remains Styela plicata
3.2.1.52 p-chloromercuribenzoate with 1 mM 20% of activity remains Styela plicata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 66000
-
 SDS-PAGE Styela plicata
3.2.1.52 132000
-
 gel filtration Styela plicata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Styela plicata
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Styela plicata
-
 sea-squirt
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 1560fold, fairly homogenous Styela plicata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 viscus
-
 Styela plicata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 67
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Styela plicata

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 0°C, in 10% glycerol, pH 5.0-6.0, no loss of activity in 4 months Styela plicata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 asialodegalactosyl fetuin glycopeptide + H2O
-
 Styela plicata N-acetylglucosamine + ?
-
 ?
3.2.1.52 asialodegalactosyl ovomucoid glycopeptide + H2O
-
 Styela plicata N-acetylglucosamine + ?
-
 ?
3.2.1.52 defucosyl Gi-x glycopeptide + H2O poor substrate Styela plicata N-acetylglucosamine + ?
-
 ?
3.2.1.52 large fragment of hyaluronic acid + H2O prepared by hyaluronidase treatment Styela plicata N-acetylglucosamine + ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Styela plicata N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Styela plicata 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Styela plicata 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 small fragment of hyaluronic acid + H2O prepared by hyaluronidase treatment, poor substrate Styela plicata N-acetylglucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 2 * 66000, SDS-PAGE Styela plicata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Styela plicata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4
-
 unstable at pH 4.0, i.e. the enzyme' pH optimum Styela plicata
3.2.1.52 5 6 stable Styela plicata