Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dewji, N.N.; De-Keyser, D.R.; Stirling, J.L.
    Purification and characterization of beta-N-acetylhexosaminidase I2 from human liver (1986), Biochem. J., 234, 157-162.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.74
-
 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside hexosaminidase B Homo sapiens
3.2.1.52 0.8
-
 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside hexosaminidase A Homo sapiens
3.2.1.52 1
-
 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside hexosaminidase I2 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 29000
-
 hexosaminidase B Homo sapiens
3.2.1.52 56000
-
 hexosaminidase A, major components: 1 * 56000 + 1 * 29000, minor components: 26000 - 22000, SDS-PAGE Homo sapiens
3.2.1.52 100000
-
 hexosaminidase A Homo sapiens
3.2.1.52 100000 110000 hexosaminidase I2, gradient gel electrophoresis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Homo sapiens
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 hexosaminidase A, B and I2 purified 2699, 10320 and 13077fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 130
-
 hexosaminidase I2, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Homo sapiens
3.2.1.52 150
-
 hexosaminidase A, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Homo sapiens
3.2.1.52 320
-
 hexosaminidase B, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -70°C, hexosaminidase A, B and I2 separated from each other Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Homo sapiens N-acetyl-D-hexosamines
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer hexosaminidase I2, major components: 1* 56000 + 1 + 29000, minor components: 65000 - 57000, SDS-PAGE Homo sapiens
3.2.1.52 dimer hexosaminidase A, major components: 1 * 56000 + 1 * 29000, minor components: 26000 - 22000, SDS-PAGE Homo sapiens