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Literature summary extracted from

  • Harley, S.M.; Beevers, L.
    Isozymes of beta-N-acetylhexosaminidase from pea seeds (Pisum sativum L.) (1987), Plant Physiol., 85, 1118-1122.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 2-mercaptoethanol blocks inhibition by Hg2+-ions Pisum sativum
3.2.1.52 Ca2+ beta-NAHA-II Pisum sativum
3.2.1.52 cysteine prevents inhibition by Hg2+-ions Pisum sativum
3.2.1.52 Mg2+ beta-NAHA-II Pisum sativum
3.2.1.52 Mn2+ beta-NAHA-II Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Cu2+ all 4 isozymes Pisum sativum
3.2.1.52 Fe2+ 2 mM causes 30-35% loss of activity of beta-NAHA-IA, 10 mM causes 30-35% loss of activity of beta-NAHA-IB Pisum sativum
3.2.1.52 Hg2+ all 4 isozymes, at 1 mM Pisum sativum
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside only beta-NAHA-IA, above 3.5 mM Pisum sativum
3.2.1.52 Zn2+ all 4 isozymes, at 10 mM inhibition ranges from 20% for beta-NAHA-IC to 45% for beta-NAHA-II Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.27
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside beta-NAHA-IA Pisum sativum
3.2.1.52 0.34
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside beta-NAHA-IB Pisum sativum
3.2.1.52 0.49
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside beta-NAHA-IA Pisum sativum
3.2.1.52 0.51
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside beta-NAHA-IB Pisum sativum
3.2.1.52 0.58
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside beta-NAHA-II Pisum sativum
3.2.1.52 0.61
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside beta-NAHA-II Pisum sativum
3.2.1.52 0.76
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside beta-NAHA-IC Pisum sativum
3.2.1.52 1.71
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside beta-NAHA-IC Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 protein body
-
 Pisum sativum 42735
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 62000
-
 beta-NAHA-II, 1 * 62000 + 1 * 64000 Pisum sativum
3.2.1.52 64000
-
 beta-NAHA-II, 1 * 62000 + 1 * 64000 Pisum sativum
3.2.1.52 210000
-
 gel filtration, all 4 isozymes Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Pisum sativum
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Pisum sativum
-
 pea
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein
-
 Pisum sativum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 4 isozymes, beta-NAHA-II was purified 3200fold to apparent homogeneity, beta-NAHA-IA 770fold, beta-NAHA-IB 1750fold, beta-NAHA-IC 500fold Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 cotyledon
-
 Pisum sativum
-
3.2.1.52 seed
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 2.08
-
 beta-NAHA-IC, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Pisum sativum
3.2.1.52 3.17
-
 beta-NAHA-IA, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Pisum sativum
3.2.1.52 7.25
-
 beta-NAHA-IB, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Pisum sativum
3.2.1.52 13.4
-
 beta-NAHA-II, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Pisum sativum

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, lyophilized Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Pisum sativum N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Pisum sativum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Pisum sativum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer beta-NAHA-II, 1 * 62000 + 1 * 64000 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.5 5 all 4 isozymes, substrates: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside and p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 6
-
 beta-NAHA-II, no hydrolysis at pH 6.0 and above, beta-NAHA-IA, -IB and IC show 20-25% of maximum activity at pH 6.0 Pisum sativum
3.2.1.52 6
-
 beta-NAHA-II, no hydrolysis above at pH 6.0 and above, beta-NAHA-IA, -IB and IC show 20-25% of maximum activity at pH 6.0 Pisum sativum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 7
-
 loss of 25% of acivity if pH is raised to 8.0 Pisum sativum