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Literature summary extracted from

  • Romaniec, M.P.M.; Fauth, U.; Kobayashi, T.; Huskisson, N.S.; Barker, P.J.; Demain, A.L.
    Purification and characterization of a new endoglucanase from Clostridium thermocellum (1992), Biochem. J., 283, 69-73.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.4 MgCl2 slightly enhances thermal stability Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 cellobiose
-
 Acetivibrio thermocellus
3.2.1.4 PCMB
-
 Acetivibrio thermocellus
3.2.1.4 SDS
-
 Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ stimulates Acetivibrio thermocellus
3.2.1.4 Mg2+ stimulates Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 76000
-
 x * 76000, SDS-PAGE Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Acetivibrio thermocellus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 no modification contains no carbohydrate Acetivibrio thermocellus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Acetivibrio thermocellus

Storage Stability

EC Number Storage Stability Organism
3.2.1.4 -20°C, stable for at least 6 months Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Acetivibrio thermocellus additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Acetivibrio thermocellus additional information
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Acetivibrio thermocellus 2 cellobiose
-
 ?
3.2.1.4 cellotriose + H2O no hydrolysis Acetivibrio thermocellus D-glucose + cellobiose
-
 ?
3.2.1.4 cellulose + H2O little ability to hydrolyze ordered cellulose Acetivibrio thermocellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 76000, SDS-PAGE Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 additional information
-
 MgCl2 slightly enhances thermal stability Acetivibrio thermocellus
3.2.1.4 60
-
 48h, 50% loss of activity Acetivibrio thermocellus
3.2.1.4 80
-
 15 min, 10% loss of activity Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 7
-
-
 Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 8 pH 5.0: about 35% of maximal activity, pH 8.0: about 30% of maximal activity Acetivibrio thermocellus