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Literature summary extracted from

  • Akiba, S.; Kimura, Y.; Yamamoto, K.; Kumagai, H.
    Purification and characterization of a protease-resistant cellulase from Aspergillus niger (1995), J. Ferment. Bioeng., 79, 125-130.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.4 the enzyme is very strongly protease resistant, not affected by 2.5 mg/ml savinase, 250 mg/l proteinase K, 250 mg/l pronase E, and 2.5 mg/ml trypsin in 50 mM Tris buffer, pH 7.5, after 3 days. 70% loss of activity after incubation with savinase for 2 weeks Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ 2 mM Aspergillus niger
3.2.1.4 Hg2+
-
 Aspergillus niger
3.2.1.4 additional information more than 50% of the original activity is retained after incubation with sodium alkyl sulfonate, sodium alkyl sulfate, polyoxyethylene alkyl sulfonate, alpha-olefin sulfonate Aspergillus niger
3.2.1.4 N-bromosuccinimide
-
 Aspergillus niger
3.2.1.4 Sn2+ 2 mM Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 40000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus niger
-
 IFO31125
-
3.2.1.4 Aspergillus niger IFO31125
-
 IFO31125
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein contains 8.9% carbohydrate Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 194
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Aspergillus niger additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Aspergillus niger IFO31125 additional information
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Aspergillus niger ?
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Aspergillus niger IFO31125 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 40000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
 2 h, 30% loss of activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6 7
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 8 more than 50% of maximal activity at pH 4.0 and at pH 8.0 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 10 40°C, stable for at least 24 h Aspergillus niger