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Literature summary extracted from

  • Sanwal, S.G.G.
    Purification and characterization of a cellulase from Catharanthus roseus stems (1999), Phytochemistry, 52, 7-13.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ 5 mM, about 30% inhibition Catharanthus roseus
3.2.1.4 EDTA 5 mM, 40% inhibition Catharanthus roseus
3.2.1.4 Hg2+ 5 mM, 95% inhibition Catharanthus roseus
3.2.1.4 Mn2+ 5 mM, about 30% inhibition Catharanthus roseus
3.2.1.4 NH4+ 5 mM, 10% inhibition Catharanthus roseus
3.2.1.4 Teepol detergent, 0.1%, 80% inhibition Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Catharanthus roseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Fe3+ 5 mM, 15% activation Catharanthus roseus
3.2.1.4 KCl 5 mM, 15% stimulation Catharanthus roseus
3.2.1.4 NaCl 5 mM, 15% stimulation Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 25000
-
 4 * 25000, SDS-PAGE Catharanthus roseus
3.2.1.4 96000
-
 gel filtration Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Catharanthus roseus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein contains 7% carbohydrate Catharanthus roseus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Catharanthus roseus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 additional information
-
-
 Catharanthus roseus

Storage Stability

EC Number Storage Stability Organism
3.2.1.4 0-4°C, stable for at least 15 days Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Catharanthus roseus additional information
-
 ?
3.2.1.4 cellulose + H2O
-
 Catharanthus roseus ?
-
 ?
3.2.1.4 additional information hydrolysis of filter paper Catharanthus roseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 tetramer 4 * 25000, SDS-PAGE Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 30 35
-
 Catharanthus roseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 5 min, stable Catharanthus roseus
3.2.1.4 60
-
 5 min, complete loss of activity Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.2
-
-
 Catharanthus roseus