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Literature summary extracted from

  • Ohmiya, Y.; Takeda, T.; Nakamura, S.; Sakai, F.; Hayashi, T.
    Purification and properties of a wall-bound endo-1,4-beta-glucanase from suspension-cultured poplar cells (1995), Plant Cell Physiol., 36, 607-614.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Cysteine hydrochloride strong stimulation Populus x canadensis
3.2.1.4 dithiothreitol slight activation Populus x canadensis
3.2.1.4 glutathione slightly enhanced Populus x canadensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ 1 mM CuCl2, 55% inhibition Populus x canadensis
3.2.1.4 EDTA 1 mM, 55% inhibition Populus x canadensis
3.2.1.4 Fe2+ 1 mM FeCl2, 13% inhibition Populus x canadensis
3.2.1.4 Hg2+ 1 mM HgCl2, complete inhibition Populus x canadensis
3.2.1.4 Mn2+ 1 mM MnCl2, 18% inhibition Populus x canadensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Populus x canadensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 cell wall bound to Populus x canadensis 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+
-
 Populus x canadensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 47000
-
 1 * 47000, SDS-PAGE Populus x canadensis
3.2.1.4 48000
-
 gel filtration Populus x canadensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Populus x canadensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Populus x canadensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 cell suspension culture
-
 Populus x canadensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 additional information
-
-
 Populus x canadensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Populus x canadensis additional information
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Populus x canadensis ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Populus x canadensis ?
-
 ?
3.2.1.4 xylan + H2O
-
 Populus x canadensis additional information
-
 ?
3.2.1.4 xyloglucan + H2O
-
 Populus x canadensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 47000, SDS-PAGE Populus x canadensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5
-
-
 Populus x canadensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 6 7.5 4°C, stable for at least 1 month in 10 mM phosphate buffer Populus x canadensis