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Literature summary extracted from

  • Lee, T.K.; Kim, C.H.
    Molecular cloning and expression of an endo-beta-1,4-D-glucanase I (avicelase I) gene from Bacillus cellulolyticus K-12 and characterization of the recombinant enzyme (1999), Appl. Biochem. Biotechnol., 80, 121-125.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Bacillus cellulyticus K-12

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 cellobiose inhibits hydrolysis of carboxymethylcellulose and avicel Bacillus cellulyticus K-12
3.2.1.4 Cu2+ CuCl2 Bacillus cellulyticus K-12
3.2.1.4 Hg2+ HgCl2 Bacillus cellulyticus K-12
3.2.1.4 PCMB
-
 Bacillus cellulyticus K-12
3.2.1.4 SDS 10 mM, 98% inhibition Bacillus cellulyticus K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Bacillus cellulyticus K-12

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ stimulates Bacillus cellulyticus K-12
3.2.1.4 Mg2+ stimulates hydrolysis of avicel and carboxymethylcellulose Bacillus cellulyticus K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 64000
-
 1 * 64000, SDS-PAGE Bacillus cellulyticus K-12
3.2.1.4 65000
-
 gel filtration Bacillus cellulyticus K-12

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus cellulyticus K-12
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Bacillus cellulyticus K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-methylumbelliferyl cellobioside + H2O
-
 Bacillus cellulyticus K-12 ?
-
 ?
3.2.1.4 avicel + H2O
-
 Bacillus cellulyticus K-12 ? cellobiose + cellotetraose ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Bacillus cellulyticus K-12 additional information
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Bacillus cellulyticus K-12 additional information cellobiose + cellotetraose ?
3.2.1.4 cellopentaose + H2O
-
 Bacillus cellulyticus K-12 additional information
-
 ?
3.2.1.4 additional information hydrolysis of filter paper Bacillus cellulyticus K-12 ?
-
 ?
3.2.1.4 p-nitrophenyl beta-D-cellobioside + H2O
-
 Bacillus cellulyticus K-12 glucose + cellobiose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 64000, SDS-PAGE Bacillus cellulyticus K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Bacillus cellulyticus K-12

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 55
-
 48 h, stable Bacillus cellulyticus K-12
3.2.1.4 55
-
 22% loss of activity after 72 h Bacillus cellulyticus K-12
3.2.1.4 70
-
 30 min, 80% loss of activity Bacillus cellulyticus K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.6
-
-
 Bacillus cellulyticus K-12

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4 10 stable Bacillus cellulyticus K-12
3.2.1.4 4 10.5 stable Bacillus cellulyticus K-12