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Literature summary extracted from

  • Tomme, P.; Gilkes, N.R.; Miller Jr., R.C.; Warren, A.J.; Kilburn, D.G.
    An internal cellulose-binding domain mediates adsorption of an engineered bifunctional xylanase/cellulase (1994), Protein Eng., 7, 117-123.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA, overexpression in Escherichia coli Cellulomonas fimi

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 additional information construction of a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA. The hybrid protein behaves like the parental endoglucanase or xylanase when assayed on a number of soluble and insoluble substrates or xylans Cellulomonas fimi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Cellulomonas fimi
3.2.1.4 0.2038
-
 2',4'-dinitrophenyl-beta-D-cellobiose endoglucanase A Cellulomonas fimi
3.2.1.4 0.2085
-
 2',4'-dinitrophenyl-beta-D-cellobiose chimeric xylanase/endoglucanase Cellulomonas fimi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Cellulomonas fimi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA, expressed in Escherichia coli Cellulomonas fimi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 2',4'-dinitrophenyl-beta-D-cellobiose + H2O
-
 Cellulomonas fimi ?
-
 ?
3.2.1.4 avicel + H2O chimeric xylanase/endoglucanase Cellulomonas fimi ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 0.358
-
 2',4'-dinitrophenyl-beta-D-cellobiose chimeric xylanase/endoglucanase Cellulomonas fimi
3.2.1.4 0.397
-
 2',4'-dinitrophenyl-beta-D-cellobiose endoglucanase A Cellulomonas fimi