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Literature summary extracted from

  • Anzai, H.; Nisizawa, K.; Matsuda, K.
    Purification and characterization of a cellulase from Dolabella auricularia (1984), J. Biochem., 96, 1381-1390.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+
-
 Dolabella auricularia
3.2.1.4 Hg2+
-
 Dolabella auricularia
3.2.1.4 Mn2+
-
 Dolabella auricularia
3.2.1.4 N-bromosuccinimide 0.001 mM, complete inactivation Dolabella auricularia
3.2.1.4 SDS 0.25 mM, complete inactivation Dolabella auricularia
3.2.1.4 Zn2+
-
 Dolabella auricularia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 44000
-
 x * 44000, SDS-PAGE Dolabella auricularia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Dolabella auricularia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Dolabella auricularia

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 277.5
-
-
 Dolabella auricularia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Dolabella auricularia additional information
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Dolabella auricularia additional information cellobiose + cellotetraose ?
3.2.1.4 cellopentaose + H2O
-
 Dolabella auricularia additional information cellotetraose + glucose ?
3.2.1.4 cellotetraose + H2O
-
 Dolabella auricularia 2 cellobiose
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Dolabella auricularia ?
-
 ?
3.2.1.4 additional information when the oligosaccharides are modified by reduction with sodium borotritide, the second linkage from the reducing end becomes significantly susceptible to the enzyme and is preferentially cleaved Dolabella auricularia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 44000, SDS-PAGE Dolabella auricularia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
 10 min, stable up to Dolabella auricularia
3.2.1.4 55
-
 10 min, complete inactivation Dolabella auricularia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.3
-
-
 Dolabella auricularia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5.5 10 0°C, 12 h, stable Dolabella auricularia