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Literature summary extracted from

  • Joliff, G.; Beguin, P.; Juy, M.; Millet, J.; Ryter, A.; Poljak, R.; Aubert, J.P.
    Isolation, crystallization and properties of a new cellulase of Clostridium thermocellum overproduced in Escherichia coli (1986), Bio/Technology, 4, 896-900.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4
-
 Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 65000
-
 equilibrium sedimentation Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Acetivibrio thermocellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 428
-
 recombinant enzyme from granules produced by Escherichia coli Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O no hydrolysis Acetivibrio thermocellus ?
-
 ?
3.2.1.4 cellulose + H2O Walseth cellulose shows 1.1% of the activity with carboxymethylcellulose Acetivibrio thermocellus ?
-
 ?
3.2.1.4 lichenan + H2O 6% of the activity with carboxymethylcellulose Acetivibrio thermocellus ?
-
 ?
3.2.1.4 additional information no activity with p-nitrophenyl-beta-D-glucoside Acetivibrio thermocellus ?
-
 ?
3.2.1.4 additional information no activity with cellobiose Acetivibrio thermocellus ?
-
 ?
3.2.1.4 xylan + H2O no activity Acetivibrio thermocellus additional information
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
 denaturation above Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
 hydrolysis of carboxymethylcellulose Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4.8 8.2 more than 50% of maximal activity at pH 4.8 and at pH 8.2 Acetivibrio thermocellus