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Literature summary extracted from

  • Lachke, A.H.; Deshpande, M.V.
    Sclerotium rolfsii: status in cellulase research (1988), FEMS Microbiol. Rev., 54, 177-194.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Aspergillus niger
3.2.1.4 additional information
-
 additional information
-
 Trichoderma reesei
3.2.1.4 additional information
-
 additional information
-
 Athelia rolfsii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 22000
-
 1 * 22000 + 1 * 32000, SDS-PAGE Athelia rolfsii
3.2.1.4 28000
-
 endoglucanase B Athelia rolfsii
3.2.1.4 32000
-
 1 * 22000 + 1 * 32000, SDS-PAGE Athelia rolfsii
3.2.1.4 52000
-
 endoglucanase A Athelia rolfsii
3.2.1.4 78000
-
 endoglucanase C Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus niger
-
-
-
3.2.1.4 Athelia rolfsii
-
-
-
3.2.1.4 Sporotrichum pulverulentum
-
-
-
3.2.1.4 Talaromyces funiculosus
-
-
-
3.2.1.4 Trichoderma reesei
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein
-
 Aspergillus niger
3.2.1.4 glycoprotein
-
 Talaromyces funiculosus
3.2.1.4 glycoprotein
-
 Sporotrichum pulverulentum
3.2.1.4 glycoprotein endoglucanase A, B and C are glycoproteins Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellohexaose + H2O
-
 Aspergillus niger additional information
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Athelia rolfsii additional information cellotriose + cellobiose + cellotriose ?
3.2.1.4 cellopentaose + H2O
-
 Aspergillus niger additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Athelia rolfsii additional information cellobiose + cellotriose + glucose ?
3.2.1.4 cellotetraose + H2O
-
 Aspergillus niger 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Athelia rolfsii 2 cellobiose
-
 ?
3.2.1.4 cellulose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Sporotrichum pulverulentum ?
-
 ?
3.2.1.4 cellulose + H2O Walseth cellulose Athelia rolfsii ?
-
 ?
3.2.1.4 cellulose + H2O Walseth cellulose Talaromyces funiculosus ?
-
 ?
3.2.1.4 xylan + H2O
-
 Talaromyces funiculosus additional information
-
 ?
3.2.1.4 xylan + H2O hydrolysis with endoglucanase B, no action with endoglucanase A and C Athelia rolfsii additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 dimer 1 * 22000 + 1 * 32000, SDS-PAGE Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Talaromyces funiculosus
3.2.1.4 50
-
 endoglucanase B and C Athelia rolfsii
3.2.1.4 74
-
 endoglucanase A Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
-
 Aspergillus niger
3.2.1.4 70
-
 10 min, 20% loss of activity Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 2.8
-
 and a second optimum at pH 3.2, endoglucanase B Athelia rolfsii
3.2.1.4 3.2
-
 and a second optimum at pH 2.8, endoglucanase B Athelia rolfsii
3.2.1.4 4
-
-
 Aspergillus niger
3.2.1.4 4
-
 endoglucanase A and C Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4.5
-
 stable, endoglucanase A, B and C Athelia rolfsii