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Literature summary extracted from

  • Bhushan, B.
    Production and characterization of a thermostable chitinase from a new alkalophilic Bacillus sp. BG-11 (2000), J. Appl. Microbiol., 88, 800-808.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.14 survives 10 freezings and thawings without loss of activity. Retains 90% and 80% of its activity in presence of trypsin and rennilase respectively Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 chitobiose
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 chitotriose
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 D-galactosamine
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 D-glucosamine
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 N-acetylglucosamine
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 54.29
-
 chitin in N-acetylglucasmine equivalents Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 41000
-
 x * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus sp. (in: Bacteria)
-
 BG-11
-
3.2.1.14 Bacillus sp. (in: Bacteria) BG-11
-
 BG-11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 16fold Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
-
 Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 4°C, 8 weeks: no loss of activity, 10 weeks: 80% of activity remains in presence of NaN3, sodium metabisulfite or NaCl, 25°C, 4 weeks: no loss of activity in presence of NaN3, sodium metabisulfite or NaCl and glycerol, 8 weeks: 75% of activity remains in presence of NaN3, sodium metabisulfite or NaCl and glycerol Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O swollen chitin Bacillus sp. (in: Bacteria) N,N-diacetylchitobiose + N-acetylglucosamine N-acetylglucosamine only ?
3.2.1.14 chitin + H2O swollen chitin Bacillus sp. (in: Bacteria) BG-11 N,N-diacetylchitobiose + N-acetylglucosamine N-acetylglucosamine only ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 45 55 immobilized chitinase Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 70
-
 half life 90 min at 70°C, immobilized chitinase Bacillus sp. (in: Bacteria)
3.2.1.14 80
-
 half life 70 min at 80°C, immobilized chitinase Bacillus sp. (in: Bacteria)
3.2.1.14 90
-
 half life 60 min at 90°C, immobilized chitinase Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7.5 9 immobilized chitinase Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 4 immobilized chitinase Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 5 10 immobilized chitinase Bacillus sp. (in: Bacteria)
3.2.1.14 6 9 free enzyme Bacillus sp. (in: Bacteria)