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Literature summary extracted from

  • Sakurada, M.; Morgavi, D.P.; Komatani, K.; Tomita, Y.; Onodera, R.
    Purification and characteristics of an autolytic chitinase of Piromyces communis OTS1 from culture medium (1997), Curr. Microbiol., 35, 48-51.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 allosamidin
-
 Piromyces communis
3.2.1.14 EDTA
-
 Piromyces communis
3.2.1.14 Hg2+
-
 Piromyces communis
3.2.1.14 phenylmethanesulfonylflouride
-
 Piromyces communis
3.2.1.14 SDS
-
 Piromyces communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Piromyces communis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 44900
-
 x * 44900, SDS-PAGE Piromyces communis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Piromyces communis
-
 OTS1
-
3.2.1.14 Piromyces communis OTS1
-
 OTS1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 24.06fold Piromyces communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Piromyces communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
 1004.33 pkat/mg Piromyces communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
-
 Piromyces communis ? + 4-methylumbelliferone
-
 ?
3.2.1.14 4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
-
 Piromyces communis OTS1 ? + 4-methylumbelliferone
-
 ?
3.2.1.14 cell wall + H2O fungal cell wall Piromyces communis chitin oligosaccharides
-
 ?
3.2.1.14 cell wall + H2O fungal cell wall Piromyces communis OTS1 chitin oligosaccharides
-
 ?
3.2.1.14 chitin + H2O colloidal chitin: trace amounts of product, regenerated chitin: slowly, powdered chitin: rapidly Piromyces communis N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?
3.2.1.14 chitin + H2O colloidal chitin: trace amounts of product, regenerated chitin: slowly, powdered chitin: rapidly Piromyces communis OTS1 N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?
3.2.1.14 glycol chitin + H2O slowly Piromyces communis N-acetylglucosamine + ?
-
 ?
3.2.1.14 glycol chitin + H2O slowly Piromyces communis OTS1 N-acetylglucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 44900, SDS-PAGE Piromyces communis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 40 60 50% of activity remains between 40 and 60°C Piromyces communis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
-
 Piromyces communis
3.2.1.14 70
-
 enzyme inactivated at 70°C Piromyces communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 5 8
-
 Piromyces communis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 6 8
-
 Piromyces communis