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Literature summary extracted from

  • Bhushan, B.; Hoondal, G.S.
    Isolation, purification and properties of a thermostable chitinase from an alkalphilic Bacillus sp. BG-11 (1998), Biotechnol. Lett., 20, 157-159.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.14 SDS
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 sodium deoxycholate
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 sodium taurocholate
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Triton X-100
-
 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 2-mercaptoethanol
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Ag+
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 AsO2-
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Cd2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Co2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 dithiothreitol
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 EDTA
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 glutathione
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Hg2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 iodoacetamide
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 iodoacetic acid
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 NaCl 50% inhibition at 7% NaCl Bacillus sp. (in: Bacteria)
3.2.1.14 phenylmethanesulfonylflouride
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Sr2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 54.29
-
 chitin in N-acetylglucasmine equivalents Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ stimulating agent Bacillus sp. (in: Bacteria)
3.2.1.14 Ni2+ stimulating agent Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus sp. (in: Bacteria)
-
 BG-11
-
3.2.1.14 Bacillus sp. (in: Bacteria) BG-11
-
 BG-11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 16.5fold Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 126.7
-
 swollen chitin Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 4°C, 60 days stable Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O swollen chitin Bacillus sp. (in: Bacteria) N,N-diacetylchitobiose + N-acetylglucosamine N-acetylglucosamine only ?
3.2.1.14 chitin + H2O swollen chitin Bacillus sp. (in: Bacteria) BG-11 N,N-diacetylchitobiose + N-acetylglucosamine N-acetylglucosamine only ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 45 55
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 two hours at pH 6-9 Bacillus sp. (in: Bacteria)
3.2.1.14 60
-
 50% activity remains after 90 min Bacillus sp. (in: Bacteria)
3.2.1.14 70
-
 50% activity remains after 30 min Bacillus sp. (in: Bacteria)
3.2.1.14 80
-
 50% activity remains after 20 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7.5 9
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 6 9 two hours at 50°C Bacillus sp. (in: Bacteria)