BRENDA - Enzyme Database

Purification, properties, and partial amino acid sequence of chitinase from a marine Alteromonas sp. strain O-7

Tsujibo, H.; Yoshida, Y.; Miyamoto, K.; Imada, C.; Okami, Y.; Inamori, Y.; Can. J. Microbiol. 38, 891-897 (1992) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.14
2-Hydroxy-5-nitrobenzyl bromide
-
Alteromonas sp.
3.2.1.14
Fe2+
-
Alteromonas sp.
3.2.1.14
Fe3+
-
Alteromonas sp.
3.2.1.14
N-bromosuccinimide
-
Alteromonas sp.
3.2.1.14
Zn2+
-
Alteromonas sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.14
extracellular
-
Alteromonas sp.
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.14
Ca2+
activate enzyme
Alteromonas sp.
3.2.1.14
K+
activates enzyme
Alteromonas sp.
3.2.1.14
Mg2+
activates enzyme significantly
Alteromonas sp.
3.2.1.14
Mn2+
slightly stimulates activity
Alteromonas sp.
3.2.1.14
Na+
activates enzyme
Alteromonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.14
70000
-
x * 70000, SDS-PAGE
Alteromonas sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.14
Alteromonas sp.
-
-
-
3.2.1.14
Alteromonas sp. O-7
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.14
4.5fold
Alteromonas sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.14
culture filtrate
-
Alteromonas sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.14
4.5
-
-
Alteromonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.14
chitohexaose + H2O
-
393526
Alteromonas sp.
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
chitohexaose + H2O
-
393526
Alteromonas sp. O-7
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp. O-7
?
3.2.1.14
chitopentaose + H2O
-
393526
Alteromonas sp.
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
chitopentaose + H2O
-
393526
Alteromonas sp. O-7
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp. O-7
?
3.2.1.14
chitotetraose + H2O
-
393526
Alteromonas sp.
N-acetylglucosamine + chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
chitotetraose + H2O
-
393526
Alteromonas sp. O-7
N-acetylglucosamine + chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp. O-7
?
3.2.1.14
N,N',N''-triacetylchitotriose + H2O
-
393526
Alteromonas sp.
N-acetylglucosamine + N,N'-diacetylchitobiose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
N,N',N''-triacetylchitotriose + H2O
-
393526
Alteromonas sp. O-7
N-acetylglucosamine + N,N'-diacetylchitobiose
chitobiose only
393526
Alteromonas sp. O-7
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.14
?
x * 70000, SDS-PAGE
Alteromonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.14
50
-
-
Alteromonas sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.14
60
-
inactivated at 60°C
Alteromonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.14
8
-
-
Alteromonas sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.14
5
10
-
Alteromonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.14
2-Hydroxy-5-nitrobenzyl bromide
-
Alteromonas sp.
3.2.1.14
Fe2+
-
Alteromonas sp.
3.2.1.14
Fe3+
-
Alteromonas sp.
3.2.1.14
N-bromosuccinimide
-
Alteromonas sp.
3.2.1.14
Zn2+
-
Alteromonas sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.14
extracellular
-
Alteromonas sp.
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.14
Ca2+
activate enzyme
Alteromonas sp.
3.2.1.14
K+
activates enzyme
Alteromonas sp.
3.2.1.14
Mg2+
activates enzyme significantly
Alteromonas sp.
3.2.1.14
Mn2+
slightly stimulates activity
Alteromonas sp.
3.2.1.14
Na+
activates enzyme
Alteromonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.14
70000
-
x * 70000, SDS-PAGE
Alteromonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.14
4.5fold
Alteromonas sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.14
culture filtrate
-
Alteromonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.14
4.5
-
-
Alteromonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.14
chitohexaose + H2O
-
393526
Alteromonas sp.
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
chitohexaose + H2O
-
393526
Alteromonas sp. O-7
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp. O-7
?
3.2.1.14
chitopentaose + H2O
-
393526
Alteromonas sp.
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
chitopentaose + H2O
-
393526
Alteromonas sp. O-7
chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp. O-7
?
3.2.1.14
chitotetraose + H2O
-
393526
Alteromonas sp.
N-acetylglucosamine + chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
chitotetraose + H2O
-
393526
Alteromonas sp. O-7
N-acetylglucosamine + chitobiose + chitotriose
chitobiose only
393526
Alteromonas sp. O-7
?
3.2.1.14
N,N',N''-triacetylchitotriose + H2O
-
393526
Alteromonas sp.
N-acetylglucosamine + N,N'-diacetylchitobiose
chitobiose only
393526
Alteromonas sp.
?
3.2.1.14
N,N',N''-triacetylchitotriose + H2O
-
393526
Alteromonas sp. O-7
N-acetylglucosamine + N,N'-diacetylchitobiose
chitobiose only
393526
Alteromonas sp. O-7
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.14
?
x * 70000, SDS-PAGE
Alteromonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.14
50
-
-
Alteromonas sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.14
60
-
inactivated at 60°C
Alteromonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.14
8
-
-
Alteromonas sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.14
5
10
-
Alteromonas sp.