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Literature summary extracted from

  • Ohtakara, A.; Mitsutomi, M.; Uchida, Y.
    Purification and some properties of chitinase from Vibrio sp. (1979), J. Ferment. Technol., 57, 169-177.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+
-
 Vibrio sp.
3.2.1.14 Cu2+
-
 Vibrio sp.
3.2.1.14 EDTA
-
 Vibrio sp.
3.2.1.14 Hg2+
-
 Vibrio sp.
3.2.1.14 Sn2+
-
 Vibrio sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Vibrio sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 60000
-
 gel filtration Vibrio sp.
3.2.1.14 63000
-
 SDS-PAGE Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Vibrio sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 to homogeneity, 19.3fold Vibrio sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Vibrio sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 1.32
-
-
 Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal and powdered chitin Vibrio sp. N,N-diacetylchitobiose + N-acetylglucosamine chitobiose, chitotriose and N-acetylglucosamine ?
3.2.1.14 chitotetraose + H2O
-
 Vibrio sp. N-acetylglucosamine + chitobiose + chitotriose
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Vibrio sp. N-acetylglucosamine + ?
-
 ?
3.2.1.14 glycol chitodextrin + H2O slow Vibrio sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 63000, SDS-PAGE Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
 stable below 40°C Vibrio sp.
3.2.1.14 60
-
 unstable above 60°C Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6 8 colloidal chitin Vibrio sp.
3.2.1.14 10.5
-
 glycol chitin Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 5 11
-
 Vibrio sp.