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Literature summary extracted from
- Purification and characterization of chitinase from the stable fly, Stomoxys calcitrans (1982), Arch. Biochem. Biophys., 216, 314-321.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | Co2+ | - |
Stomoxys calcitrans |  |
| 3.2.1.14 | Cu2+ | - |
Stomoxys calcitrans | |
| 3.2.1.14 | Fe2+ | - |
Stomoxys calcitrans | |
| 3.2.1.14 | Hg2+ | - |
Stomoxys calcitrans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 33 | - |
chitin | expressed in N-acetylglucosamine equivalents | Stomoxys calcitrans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 48000 | - |
x * 48000, SDS-PAGE | Stomoxys calcitrans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Stomoxys calcitrans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | to homogeneity | Stomoxys calcitrans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | pupa | - |
Stomoxys calcitrans | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 0.7189 | - |
- |
Stomoxys calcitrans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.14 | -20°C, prolonged storage, repeated freezing and thawing, no loss of activity | Stomoxys calcitrans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | chitin + H2O | [acetyl-3H]chitin | Stomoxys calcitrans | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 48000, SDS-PAGE | Stomoxys calcitrans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 5 | - |
- |
Stomoxys calcitrans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 5.5 | 7 | between pH 5.5 and 7, 80% activity | Stomoxys calcitrans |
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