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Literature summary extracted from

  • Cabib, E.
    Endochitinase from wheat germ (1988), Methods Enzymol., 161, 498-501.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 2
-
 chitin chitin concentration calculated as N-acetylglucosamine Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 29000
-
 gel filtration Triticum aestivum
3.2.1.14 30000
-
 SDS-PAGE Triticum aestivum
3.2.1.14 33000
-
 sedimentation equilibrium Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 14.6
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 -20°C or 2°C, several weeks, no loss of activity Triticum aestivum
3.2.1.14 -80°C, 7 years, no loss of activity Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Triticum aestivum N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?
3.2.1.14 chitosan + H2O reacetylated Triticum aestivum chitosan oligosaccharides from diacetylchitobiose to tetraacetylchitotetraose ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 9
-
 Triticum aestivum