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Literature summary extracted from

  • Kato, K.; Sugimoto, T.; Amemura, A.; Harada, T.
    A Pseudomonas intracellular amylase with high activity on maltodextrins and cyclodextrins (1975), Biochim. Biophys. Acta, 391, 96-108.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ag+ 2 mM, complete inhibition Pseudomonas sp.
3.2.1.1 Cd2+ 2 mM, 65% inhibition Pseudomonas sp.
3.2.1.1 Cu2+ 2 mM, complete inhibition Pseudomonas sp.
3.2.1.1 Fe2+ 2 mM, 49% inhibition Pseudomonas sp.
3.2.1.1 Hg2+ 2 mM, complete inhibition Pseudomonas sp.
3.2.1.1 Pb2+ 2 mM, 53% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information
-
 Pseudomonas sp.
3.2.1.1 2
-
 maltotriose
-
 Pseudomonas sp.
3.2.1.1 2.1
-
 maltotetraose
-
 Pseudomonas sp.
3.2.1.1 3.6
-
 cyclomaltohexaose
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 intracellular
-
 Pseudomonas sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 96000
-
 x * 96000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pseudomonas sp.
-
 MS1
-
3.2.1.1 Pseudomonas sp. MS1
-
 MS1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 21
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 Pseudomonas sp. additional information
-
 ?
3.2.1.1 amylopectin + H2O
-
 Pseudomonas sp. MS1 additional information
-
 ?
3.2.1.1 amylose + H2O
-
 Pseudomonas sp. additional information
-
 ?
3.2.1.1 amylose + H2O
-
 Pseudomonas sp. MS1 additional information
-
 ?
3.2.1.1 cyclomaltohexaose + H2O
-
 Pseudomonas sp. ?
-
 ?
3.2.1.1 cyclomaltohexaose + H2O
-
 Pseudomonas sp. MS1 ?
-
 ?
3.2.1.1 maltopentaose + H2O
-
 Pseudomonas sp. additional information glucose + maltose + maltotriose + a slight amount of maltotetraose ?
3.2.1.1 maltotetraose + H2O
-
 Pseudomonas sp. additional information glucose + maltose + maltotriose ?
3.2.1.1 maltotriose + H2O
-
 Pseudomonas sp. maltose + D-glucose
-
 ?
3.2.1.1 maltotriose + H2O
-
 Pseudomonas sp. MS1 maltose + D-glucose
-
 ?
3.2.1.1 starch + H2O soluble starch Pseudomonas sp. additional information
-
 ?
3.2.1.1 starch + H2O soluble starch Pseudomonas sp. MS1 additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 96000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 50
-
 30 min, stable Pseudomonas sp.
3.2.1.1 60
-
 30 min, complete loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5
-
-
 Pseudomonas sp.