Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Allam, A.M.; Hussein, A.M.; Ragab, A.M.
    Amylase of the thermophilic actinomycete Thermomonospora vulgaris (1975), Z. Allg. Mikrobiol., 15, 393-398.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.1 bovine serum albumin protects from thermal denaturation at 60°C, no protection at 80°C Thermomonospora vulgaris
3.2.1.1 stable in presence of 8 M urea Thermomonospora vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Cd2+ 1 mM CdSO4, 82% inhibition Thermomonospora vulgaris
3.2.1.1 Cu2+ 1 mM CuSO4, 82% inhibition Thermomonospora vulgaris
3.2.1.1 EDTA inhibition is completely nullified by Ca2+ Thermomonospora vulgaris
3.2.1.1 Fe2+ 10 mM, 86% inhibition Thermomonospora vulgaris
3.2.1.1 iodoacetate
-
 Thermomonospora vulgaris
3.2.1.1 Mg2+ 10 mM MgCl2, 86% inhibition Thermomonospora vulgaris
3.2.1.1 Mn2+ 1 mM MnSO4, 84% inhibition Thermomonospora vulgaris
3.2.1.1 Zn2+ 10 mM, 84% inhibition Thermomonospora vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information
-
 Thermomonospora vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 KCN 10 mM, 82% inhibition Thermomonospora vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Thermomonospora vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 partial Thermomonospora vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 60
-
-
 Thermomonospora vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 35 70 35°C: about 35% of maximal activity, 70°C: about 75% of maximal activity Thermomonospora vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 additional information
-
 bovine serum albumin protects from thermal denaturation at 60°C, no protection at 80°C Thermomonospora vulgaris
3.2.1.1 80
-
 complete inactivation after a few min Thermomonospora vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5 8 pH 5.0: about 35% of maximal activity, pH 8.0: about 30% of maximal activity Thermomonospora vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 3
-
 few min, complete inactivation Thermomonospora vulgaris