Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kindle, K.L.
    Characterization and production of thermostable alpha-amylase (1983), Appl. Biochem. Biotechnol., 8, 153-170.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Bacillus subtilis Bacillus subtilis
3.2.1.1 expression in Bacillus subtilis Bacillus amyloliquefaciens
3.2.1.1 expression in Escherichia coli Priestia megaterium
3.2.1.1 expression in Escherichia coli Weizmannia coagulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 EDTA
-
 Bacillus licheniformis
3.2.1.1 EDTA
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 EDTA
-
 Bacillus subtilis
3.2.1.1 EDTA
-
 Geobacillus stearothermophilus
3.2.1.1 EDTA
-
 Thermomonospora vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+
-
 Bacillus subtilis
3.2.1.1 Ca2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 Ca2+ required Alicyclobacillus acidocaldarius
3.2.1.1 Ca2+ stimulates Geobacillus stearothermophilus
3.2.1.1 Ca2+ stimulates Bacillus licheniformis
3.2.1.1 Mg2+
-
 Bacillus subtilis
3.2.1.1 Mg2+
-
 Bacillus licheniformis
3.2.1.1 Mg2+ required Alicyclobacillus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 22500
-
 gel filtration Bacillus licheniformis
3.2.1.1 41000
-
 gel filtration Geobacillus stearothermophilus
3.2.1.1 44000 48000
-
 Geobacillus stearothermophilus
3.2.1.1 44000 48000 sedimentation measurement Humicola stellata
3.2.1.1 54000
-
 x * 54000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.1 68000
-
 x * 68000, SDS-PAGE Bacillus subtilis
3.2.1.1 68000
-
 x * 68000, SDS-PAGE Alicyclobacillus acidocaldarius
3.2.1.1 90000
-
 x * 90000, SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Thermomonospora vulgaris enzyme production is induced by 2% starch or solid CaCO3 glucooligosaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Acinetobacter sp.
-
-
-
3.2.1.1 Alicyclobacillus acidocaldarius
-
-
-
3.2.1.1 Aspergillus niger
-
-
-
3.2.1.1 Bacillus amyloliquefaciens
-
-
-
3.2.1.1 Bacillus licheniformis
-
-
-
3.2.1.1 Bacillus licheniformis CUMC305
-
-
-
3.2.1.1 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.1 Bacillus sp. (in: Bacteria) 11-15
-
-
-
3.2.1.1 Bacillus subtilis
-
-
-
3.2.1.1 Geobacillus stearothermophilus
-
-
-
3.2.1.1 Humicola brevis
-
-
-
3.2.1.1 Humicola insolens
-
-
-
3.2.1.1 Humicola stellata
-
-
-
3.2.1.1 Malbranchea pulchella
-
-
-
3.2.1.1 Micromonospora vulgaris
-
-
-
3.2.1.1 Mycothermus thermophilus
-
-
-
3.2.1.1 Priestia megaterium
-
-
-
3.2.1.1 Rhizomucor miehei
-
-
-
3.2.1.1 Rhizomucor pusillus
-
-
-
3.2.1.1 Rhizopus microsporus
-
-
-
3.2.1.1 Thermoactinomyces vulgaris
-
-
-
3.2.1.1 Thermoascus aurantiacus
-
-
-
3.2.1.1 Thermoascus crustaceus
-
-
-
3.2.1.1 Thermochaetoides thermophila
-
-
-
3.2.1.1 Thermomonospora curvata
-
-
-
3.2.1.1 Thermomonospora viridis
-
-
-
3.2.1.1 Thermomonospora vulgaris
-
-
-
3.2.1.1 Thermomyces dupontii
-
-
-
3.2.1.1 Thermomyces lanuginosus
-
-
-
3.2.1.1 Thermothelomyces myriococcoides
-
-
-
3.2.1.1 Trichocladium griseum
-
-
-
3.2.1.1 Weizmannia coagulans
-
-
-
3.2.1.1 Weizmannia coagulans CUMC512
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
-
 Bacillus subtilis
3.2.1.1 additional information
-
-
 Geobacillus stearothermophilus
3.2.1.1 additional information
-
-
 Weizmannia coagulans
3.2.1.1 additional information
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 additional information
-
-
 Bacillus licheniformis
3.2.1.1 additional information
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 pullulan + H2O
-
 Thermoactinomyces vulgaris panose + ?
-
 ?
3.2.1.1 pullulan + H2O
-
 Thermomonospora viridis panose + ?
-
 ?
3.2.1.1 starch + H2O enzyme production is induced by 2% starch or solid CaCO3 Thermomonospora vulgaris glucooligosaccharide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ?
-
 Bacillus licheniformis
3.2.1.1 ?
-
 Thermomonospora curvata
3.2.1.1 ? x * 54000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.1 ? x * 90000, SDS-PAGE Geobacillus stearothermophilus
3.2.1.1 ? x * 68000, SDS-PAGE Bacillus subtilis
3.2.1.1 ? x * 68000, SDS-PAGE Alicyclobacillus acidocaldarius
3.2.1.1 ? x * 55000-65000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 45 55 cells grown at 35°C Geobacillus stearothermophilus
3.2.1.1 45 55 cells grown at 35°C Weizmannia coagulans
3.2.1.1 50
-
-
 Bacillus subtilis
3.2.1.1 60
-
-
 Bacillus subtilis
3.2.1.1 60
-
-
 Aspergillus niger
3.2.1.1 60
-
-
 Thermoactinomyces vulgaris
3.2.1.1 60
-
-
 Thermomyces lanuginosus
3.2.1.1 60
-
-
 Thermomonospora vulgaris
3.2.1.1 60 70 cells grown at 55°C Geobacillus stearothermophilus
3.2.1.1 60 70 cells grown at 55°C Weizmannia coagulans
3.2.1.1 65
-
-
 Bacillus subtilis
3.2.1.1 65
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 65
-
-
 Bacillus amyloliquefaciens
3.2.1.1 65
-
-
 Thermomyces lanuginosus
3.2.1.1 65
-
-
 Thermomonospora curvata
3.2.1.1 70
-
-
 Geobacillus stearothermophilus
3.2.1.1 70
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 70
-
-
 Bacillus licheniformis
3.2.1.1 70
-
-
 Thermoactinomyces vulgaris
3.2.1.1 70
-
-
 Alicyclobacillus acidocaldarius
3.2.1.1 75
-
-
 Alicyclobacillus acidocaldarius
3.2.1.1 76
-
 pH 8 Bacillus licheniformis
3.2.1.1 85
-
 at pH 8.5 Weizmannia coagulans
3.2.1.1 91
-
 at pH 9.0, strain CUMC305 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 additional information
-
-
 Geobacillus stearothermophilus
3.2.1.1 additional information
-
-
 Bacillus licheniformis
3.2.1.1 additional information
-
-
 Thermomyces lanuginosus
3.2.1.1 additional information
-
-
 Thermomonospora vulgaris
3.2.1.1 additional information
-
 substrate is required for stability at high temperatures Aspergillus niger
3.2.1.1 60
-
-
 Bacillus subtilis
3.2.1.1 60
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 60
-
-
 Thermoascus aurantiacus
3.2.1.1 60
-
-
 Thermomyces lanuginosus
3.2.1.1 60
-
 5 min, stable Bacillus licheniformis
3.2.1.1 60
-
 half-life: 5 days Alicyclobacillus acidocaldarius
3.2.1.1 60
-
 60 min, 50% loss of activity Thermomonospora vulgaris
3.2.1.1 60
-
 18% loss of activity after 10 min, 64% loss of activity after 60 min Aspergillus niger
3.2.1.1 70
-
-
 Thermomyces lanuginosus
3.2.1.1 70
-
-
 Alicyclobacillus acidocaldarius
3.2.1.1 70
-
 5 min, stable Bacillus licheniformis
3.2.1.1 70
-
 pH 2, 10 min, 40% loss of activity, 94% loss of activity after 30 min Bacillus sp. (in: Bacteria)
3.2.1.1 70
-
 15 min, 73% loss of activity Aspergillus niger
3.2.1.1 75
-
-
 Bacillus licheniformis
3.2.1.1 75
-
 40 min, stable Weizmannia coagulans
3.2.1.1 75
-
 t1/2: 2 h Alicyclobacillus acidocaldarius
3.2.1.1 80
-
-
 Weizmannia coagulans
3.2.1.1 80
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 80
-
-
 Thermomyces lanuginosus
3.2.1.1 80
-
-
 Alicyclobacillus acidocaldarius
3.2.1.1 80
-
 5 min, complete inactivation Thermomonospora vulgaris
3.2.1.1 80
-
 5 min, 55% loss of activity Bacillus licheniformis
3.2.1.1 80
-
 15 min, complete inactivation Aspergillus niger
3.2.1.1 90
-
-
 Geobacillus stearothermophilus
3.2.1.1 90
-
-
 Bacillus licheniformis
3.2.1.1 90
-
-
 Bacillus amyloliquefaciens
3.2.1.1 90
-
-
 Alicyclobacillus acidocaldarius
3.2.1.1 90
-
 60 min, 6% loss of activity Weizmannia coagulans
3.2.1.1 90
-
 5 min, 40% loss of activity, complete inactivation after 60 min Thermomonospora curvata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 2
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 3.5
-
-
 Alicyclobacillus acidocaldarius
3.2.1.1 5
-
-
 Thermoactinomyces vulgaris
3.2.1.1 5 8
-
 Bacillus licheniformis
3.2.1.1 5 7 in presence of 0.5% starch and 4 mM CaCl2 Bacillus licheniformis
3.2.1.1 5.5 6
-
 Aspergillus niger
3.2.1.1 5.5 6
-
 Thermomonospora curvata
3.2.1.1 5.9 7
-
 Thermoactinomyces vulgaris
3.2.1.1 5.9 7
-
 Thermomonospora viridis
3.2.1.1 6
-
-
 Bacillus subtilis
3.2.1.1 6
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 6
-
-
 Thermomonospora vulgaris
3.2.1.1 6.5 8
-
 Geobacillus stearothermophilus
3.2.1.1 9.5
-
 strain CUMC305 Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 additional information
-
 Ca2+ is required for stability at high pH Aspergillus niger
3.2.1.1 1 2 65°C, 15 min, stable Bacillus sp. (in: Bacteria)
3.2.1.1 3.4 6.4 optimal stability Aspergillus niger
3.2.1.1 3.8
-
 5 min, complete inactivation Thermomonospora vulgaris
3.2.1.1 6 11
-
 Geobacillus stearothermophilus
3.2.1.1 6 11 25°C, 24 h, stable Bacillus licheniformis
3.2.1.1 7
-
 60 min, stable Thermomonospora vulgaris
3.2.1.1 9
-
 30 min, 25% loss of activity Thermomonospora vulgaris