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Literature summary extracted from

  • Gogoi, B.K.; Bezbaruah, R.L.; Pillai, K.R.; Baruah, J.N.
    Production, purification and characterization of an alpha-amylase produced by Saccharomycopsis fibuligera (1987), J. Appl. Bacteriol., 63, 373-379.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 (NH4)2SO4
-
Saccharomycopsis fibuligera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
additional information 0.572 mg/ml for soluble starch Saccharomycopsis fibuligera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
Saccharomycopsis fibuligera
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 40900
-
gel filtration Saccharomycopsis fibuligera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Saccharomycopsis fibuligera high enzyme production during stationary phase of growth, lack of catabolite repression glucooligosaccharide
-
?
3.2.1.1 starch + H2O Saccharomycopsis fibuligera ST 2 high enzyme production during stationary phase of growth, lack of catabolite repression glucooligosaccharide
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Saccharomycopsis fibuligera
-
ST 2
-
3.2.1.1 Saccharomycopsis fibuligera ST 2
-
ST 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
Saccharomycopsis fibuligera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O soluble starch Saccharomycopsis fibuligera additional information glucose + maltose + maltotriose + maltotetraose ?
3.2.1.1 starch + H2O soluble starch Saccharomycopsis fibuligera ST 2 additional information glucose + maltose + maltotriose + maltotetraose ?
3.2.1.1 starch + H2O high enzyme production during stationary phase of growth, lack of catabolite repression Saccharomycopsis fibuligera glucooligosaccharide
-
?
3.2.1.1 starch + H2O high enzyme production during stationary phase of growth, lack of catabolite repression Saccharomycopsis fibuligera ST 2 glucooligosaccharide
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 60
-
-
Saccharomycopsis fibuligera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 50
-
stable for 10 min in absence of Ca2+ Saccharomycopsis fibuligera
3.2.1.1 60
-
10% loss of activity in presence of 0.00125 M Ca2+, 86% loss of activity without Ca2+ Saccharomycopsis fibuligera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 4.8 6
-
Saccharomycopsis fibuligera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 4 6.8 pH 4.0: 29% of maximal activity, pH 6.8: 23% of maximal activity Saccharomycopsis fibuligera

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 3
-
10°C, 24 h, 95% loss of activity Saccharomycopsis fibuligera
3.2.1.1 4 9.4 stable Saccharomycopsis fibuligera