Data extracted from this reference:
Cloned(Commentary)
3.13.1.1
expressed in Escherichia coli
Arabidopsis thaliana
Localization
3.13.1.1
chloroplast
Arabidopsis thaliana
9507
Natural Substrates/ Products (Substrates)
3.13.1.1
UDP-glucose + ?
Arabidopsis thaliana
first step of sulfolipid biosynthesis, unknown sulfur donor
UDP-sulfoquinovose + H2O
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
Organism
3.13.1.1
Arabidopsis thaliana
Purification (Commentary)
3.13.1.1
Ni2+-nitrilotriacetic acid column
Arabidopsis thaliana
Reaction
3.13.1.1
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
sequence similarity to sugar nucleotide modifying enzymes, Thr228 seems to be catalytically important; short-chain dehydrogenase/reductase family
Arabidopsis thaliana
Storage Stability
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days
Arabidopsis thaliana
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days
Arabidopsis thaliana
Substrates and Products (Substrate)
3.13.1.1
UDP-glucose + ?
first step of sulfolipid biosynthesis, unknown sulfur donor
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
393364
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
Cofactor
3.13.1.1
NAD+
tightly bound, enzyme binds 1 NAD+ per monomer, side-chain chemistry and hydrogen-bonding patterns in the NAD+ site
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
3.13.1.1
expressed in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
3.13.1.1
NAD+
tightly bound, enzyme binds 1 NAD+ per monomer, side-chain chemistry and hydrogen-bonding patterns in the NAD+ site
Arabidopsis thaliana
Localization (protein specific)
3.13.1.1
chloroplast
Arabidopsis thaliana
9507
Natural Substrates/ Products (Substrates) (protein specific)
3.13.1.1
UDP-glucose + ?
Arabidopsis thaliana
first step of sulfolipid biosynthesis, unknown sulfur donor
UDP-sulfoquinovose + H2O
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
Purification (Commentary) (protein specific)
3.13.1.1
Ni2+-nitrilotriacetic acid column
Arabidopsis thaliana
Storage Stability (protein specific)
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days
Arabidopsis thaliana
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
3.13.1.1
UDP-glucose + ?
first step of sulfolipid biosynthesis, unknown sulfur donor
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
393364
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O