show Prediction of the active-site structure and NAD+ binding in SQD1, a protein essential for sulfolipid biosynthesis in Arabidopsis
Essigmann, B.; Hespenheide, B.M.; Kuhn, L.A.; Benning, C.; Arch. Biochem. Biophys. 369, 30-41 (1999)
Data extracted from this reference:
Cloned(Commentary)
EC Number
Commentary
Organism
3.13.1.1
expressed in Escherichia coli
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.13.1.1
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.13.1.1
UDP-glucose + ?
Arabidopsis thaliana
first step of sulfolipid biosynthesis, unknown sulfur donor
UDP-sulfoquinovose + H2O
-
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
Arabidopsis thaliana
-
UDP-sulfoquinovose + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.13.1.1
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.13.1.1
Ni2+-nitrilotriacetic acid column
Arabidopsis thaliana
Reaction
EC Number
Reaction
Commentary
Organism
3.13.1.1
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
sequence similarity to sugar nucleotide modifying enzymes, Thr228 seems to be catalytically important; short-chain dehydrogenase/reductase family
Arabidopsis thaliana
Storage Stability
EC Number
Storage Stability
Organism
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days
Arabidopsis thaliana
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.13.1.1
UDP-glucose + ?
first step of sulfolipid biosynthesis, unknown sulfur donor
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
393364
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
-
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
3.13.1.1
NAD+
tightly bound, enzyme binds 1 NAD+ per monomer, side-chain chemistry and hydrogen-bonding patterns in the NAD+ site
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.13.1.1
expressed in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
3.13.1.1
NAD+
tightly bound, enzyme binds 1 NAD+ per monomer, side-chain chemistry and hydrogen-bonding patterns in the NAD+ site
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.13.1.1
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.13.1.1
UDP-glucose + ?
Arabidopsis thaliana
first step of sulfolipid biosynthesis, unknown sulfur donor
UDP-sulfoquinovose + H2O
-
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
Arabidopsis thaliana
-
UDP-sulfoquinovose + H2O
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.13.1.1
Ni2+-nitrilotriacetic acid column
Arabidopsis thaliana
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days
Arabidopsis thaliana
3.13.1.1
4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.13.1.1
UDP-glucose + ?
first step of sulfolipid biosynthesis, unknown sulfur donor
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
393364
Arabidopsis thaliana
?
3.13.1.1
UDP-glucose + sulfite
-
393364
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
-
-
-