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Literature summary extracted from

  • Nakao, M.; Nakayama, T.; Harada, M.; Kakudo, A.; Ikemoto, H.; Kobayashi, S.; Shibano, Y.
    Purification and characterization of a Bacillus sp. SAM1606 thermostable alpha-glucosidase with transglucosylation activity (1994), Appl. Microbiol. Biotechnol., 41, 337-343.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.20 nutrition high thermostability of the enzyme permits the transglucosylation reaction at high temperatures, which is beneficial for continous production of oligosaccharides from sucrose Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Ag+ 0.1 mM Bacillus sp. (in: Bacteria)
3.2.1.20 Cu2+ 0.1 mM Bacillus sp. (in: Bacteria)
3.2.1.20 Pb2+ 0.1 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 2.4
-
 isomaltose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 3.2
-
 trehalose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 4.1
-
 isomaltulose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 5.3
-
 sucrose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 6.7
-
 isomaltotriose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 8.7
-
 turanose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 10.5
-
 nigerose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 10.8
-
 maltose
-
 Bacillus sp. (in: Bacteria)
3.2.1.20 13.3
-
 maltotriose
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 64000
-
 x * 64000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Bacillus sp. (in: Bacteria)
-
 SAM1606
-
3.2.1.20 Bacillus sp. (in: Bacteria) SAM1606
-
 SAM1606
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant enzyme Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 81.23
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) 2 alpha-D-glucose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 2 alpha-D-glucose
-
 ?
3.2.1.20 isomaltotriose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose
-
 ?
3.2.1.20 isomaltotriose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
3.2.1.20 additional information transglucosidase activity Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.20 additional information transglucosidase activity Bacillus sp. (in: Bacteria) SAM1606 ?
-
 ?
3.2.1.20 nigerose + H2O
-
 Bacillus sp. (in: Bacteria) 2 alpha-D-glucose
-
 ?
3.2.1.20 nigerose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 2 alpha-D-glucose
-
 ?
3.2.1.20 palatinose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + D-fructose
-
 ?
3.2.1.20 sucrose
-
 Bacillus sp. (in: Bacteria) oligosaccharides containing theanderose ?
3.2.1.20 sucrose + H2O
-
 Bacillus sp. (in: Bacteria) D-fructose + alpha-D-glucose
-
 ?
3.2.1.20 trehalose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose
-
 ?
3.2.1.20 turanose + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 64000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 75
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 60 80 60°C: about 35% of maximal activity, 80°C: about 70% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 65
-
 pH 7.2, 10 min, stable up to Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 54.6
-
 sucrose
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5
-
-
 Bacillus sp. (in: Bacteria)