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Literature summary extracted from

  • Rolfsmeier, M.; Blum, P.
    Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus (1995), J. Bacteriol., 177, 482-485.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.20 n-Propanol maximal stimulation at 7.5% Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
3.2.1.20 proteolytic treatment of chemically denatured enzyme generated by guanidine hydrochloride pretreatment results in complete maltase degradation Saccharolobus solfataricus
3.2.1.20 treatment with trypsin or chymotrypsin at 30°C or proteinase K at 30°C or at 55°C for 20 min in 40 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, pH 7.6, 8 mM magnesium acetate, 0.3 mM EDTA, 2 mM dithiothreitol, 10 mM sodium chloride and 20 mM potassium chloride has no effect Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.91
-
 maltose
-
 Saccharolobus solfataricus
3.2.1.20 3.2
-
 p-nitrophenyl-alpha-D-glucoside
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 80000
-
 4 * or 5 * 80000, PAGE after treatment with 6 M guanidine hydrochloride Saccharolobus solfataricus
3.2.1.20 80000
-
 5 * or 4 * 80000, PAGE after treatment with 6 M guanidine hydrochloride Saccharolobus solfataricus
3.2.1.20 400000
-
 gel filtration Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.20 Ethanol 20 min in both 50% n-propanol and 25% ethanol increases maltase activity by over 50% Saccharolobus solfataricus
3.2.1.20 n-propanol 20 min in both 50% n-propanol and 25% ethanol increases maltase activity by over 50% Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 120900
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 dextrin + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.20 isomaltose + H2O
-
 Saccharolobus solfataricus 2 alpha-D-glucose
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Saccharolobus solfataricus maltohexaose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Saccharolobus solfataricus alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Saccharolobus solfataricus alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Saccharolobus solfataricus maltose + D-glucose
-
 ?
3.2.1.20 methyl-alpha-glucoside + H2O
-
 Saccharolobus solfataricus methanol + alpha-D-glucose
-
 ?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 starch + H2O no activity Saccharolobus solfataricus alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 pentamer 5 * or 4 * 80000, PAGE after treatment with 6 M guanidine hydrochloride Saccharolobus solfataricus
3.2.1.20 tetramer 4 * or 5 * 80000, PAGE after treatment with 6 M guanidine hydrochloride Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 105
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 65 115 65°C: about 30% of maximal activity, 115°C: about 25% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 85
-
 half-life: 11 h Saccharolobus solfataricus
3.2.1.20 95
-
 half-life: 3 h Saccharolobus solfataricus
3.2.1.20 100
-
 half-life: 2.75 h Saccharolobus solfataricus
3.2.1.20 105
-
 half-life: 11 h Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 3.5 6.5 about 50% of maximal activity at pH 3.5 and at pH 6.5 Saccharolobus solfataricus