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Literature summary extracted from
- Purification and properties of extracellular alpha-glucosidase of a thermophile, Bacillus thermoglucosidius KP 1006 (1976), Biochim. Biophys. Acta, 445, 386-397.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.20 | 45% ethanol, 7.2 M urea or 0.06% SDS, fail to inactivate the enzyme at 25°C. The tolerance is extremely reduced at 60°C | Parageobacillus thermoglucosidasius |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | Ba2+ | - |
Parageobacillus thermoglucosidasius |  |
| 3.2.1.20 | Ca2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Co2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Cu2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | D-glucono-delta-lactone | hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside, competitive | Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | D-glucose | hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside, competitive | Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Fe3+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Hg2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | His | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | isomaltose | hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside, competitive | Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Mg2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Mn2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | p-nitrophenyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Pb2+ | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Tris | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | Zn2+ | - |
Parageobacillus thermoglucosidasius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.23 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | 3 | - |
phenyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | 3.2 | - |
isomaltose | - |
Parageobacillus thermoglucosidasius | |
| 3.2.1.20 | 37 | - |
methyl-alpha-D-glucopyranoside | - |
Parageobacillus thermoglucosidasius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.20 | extracellular | - |
Parageobacillus thermoglucosidasius | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 55000 | - |
gel filtration | Parageobacillus thermoglucosidasius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Parageobacillus thermoglucosidasius | - |
- |
- |
| 3.2.1.20 | Parageobacillus thermoglucosidasius KP 1006 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | - |
Parageobacillus thermoglucosidasius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 164 | - |
- |
Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | isomaltose + H2O | - |
Parageobacillus thermoglucosidasius | 2 alpha-D-glucose | - |
? | |
| 3.2.1.20 | isomaltose + H2O | - |
Parageobacillus thermoglucosidasius KP 1006 | 2 alpha-D-glucose | - |
? | |
| 3.2.1.20 | methyl-alpha-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius | methanol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | methyl-alpha-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius KP 1006 | methanol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | p-nitrophenyl-alpha-D-glucopyranoside | Parageobacillus thermoglucosidasius | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.20 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | p-nitrophenyl-alpha-D-glucopyranoside | Parageobacillus thermoglucosidasius KP 1006 | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.20 | phenyl alpha-D-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius | phenol + alpha-D-glucose | - |
? | |
| 3.2.1.20 | phenyl alpha-D-glucoside + H2O | phenyl-alpha-glucopyranoside | Parageobacillus thermoglucosidasius KP 1006 | phenol + alpha-D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 75 | - |
- |
Parageobacillus thermoglucosidasius |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 30 | 60 | 2 h, stable | Parageobacillus thermoglucosidasius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 5 | 6 | - |
Parageobacillus thermoglucosidasius |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 4 | 11 | at 31°C, stable | Parageobacillus thermoglucosidasius |
| 3.2.1.20 | 6 | 8.5 | 55.5°C, stable | Parageobacillus thermoglucosidasius |
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