Any feedback?
Literature summary extracted from
- Purification and characterization of an alpha-glucosidase from Saccharomyces carlsbergensis (1978), Biochemistry, 17, 4657-4661.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.31 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Saccharomyces pastorianus |  |
| 3.2.1.20 | 16.6 | - |
maltose | - |
Saccharomyces pastorianus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 63000 | - |
gel filtration | Saccharomyces pastorianus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Saccharomyces pastorianus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | only a single form of enzyme | Saccharomyces pastorianus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 131.3 | - |
- |
Saccharomyces pastorianus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | maltose + H2O | - |
Saccharomyces pastorianus | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | additional information | absolute anomeric specificity for alpha-glycosidic linkages | Saccharomyces pastorianus | ? | - |
? | |
| 3.2.1.20 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Saccharomyces pastorianus | 4-nitrophenol + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | monomer | - |
Saccharomyces pastorianus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 48 | - |
decay constant: 2.42 per min | Saccharomyces pastorianus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 6.7 | 6.8 | hydrolysis of p-nitrophenyl-alpha-D-glucoside | Saccharomyces pastorianus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
