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Literature summary extracted from

  • Yamasaki, Y.; Suzuki, Y.
    Purification and properties of three forms of alpha-glucosidase from germinated green gram (Phaseolus vidissimus Ten.) (1979), Agric. Biol. Chem., 43, 481-489.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Ba2+ BaCl2 Vigna radiata
3.2.1.20 Co2+ CoCl2 Vigna radiata
3.2.1.20 Cu2+ Cu(CH3COO)2 Vigna radiata
3.2.1.20 EDTA
-
 Vigna radiata
3.2.1.20 Hg2+ HgCl2 Vigna radiata
3.2.1.20 iodoacetic acid
-
 Vigna radiata
3.2.1.20 Mn2+ MnCl2 Vigna radiata
3.2.1.20 Ni2+ NiCl2 Vigna radiata
3.2.1.20 Pb2+ Pb(CH3COO)2 Vigna radiata
3.2.1.20 PCMB
-
 Vigna radiata
3.2.1.20 Tris
-
 Vigna radiata
3.2.1.20 turanose
-
 Vigna radiata
3.2.1.20 Zn2+ ZnCl2 Vigna radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.463
-
 maltotriose alpha-glucosidase II-2 Vigna radiata
3.2.1.20 0.485
-
 maltose alpha-glucosidase II-1 Vigna radiata
3.2.1.20 0.493
-
 maltotriose alpha-glucosidase I Vigna radiata
3.2.1.20 0.584
-
 maltose alpha-glucosidase II-2 Vigna radiata
3.2.1.20 0.597
-
 maltotriose alpha-glucosidase II-1 Vigna radiata
3.2.1.20 0.714
-
 amylose alpha-glucosidase II-1 Vigna radiata
3.2.1.20 0.73
-
 maltose alpha-glucosidase I Vigna radiata
3.2.1.20 1
-
 amylose alpha-glucosidase I and II-2 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 65000
-
 x * 65000, alpha-glucosidase II-2, SDS-PAGE Vigna radiata
3.2.1.20 105000
-
 x * 105000, alpha-glucosidase II-1, SDS-PAGE Vigna radiata
3.2.1.20 145000
-
 x * 145000, alpha-glucosidase I, SDS-PAGE Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Vigna radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 glucosidase I, II-1, and II-2 Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 amylose + H2O
-
 Vigna radiata alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Vigna radiata alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Vigna radiata maltose + D-glucose
-
 ?
3.2.1.20 phenyl alpha-maltoside + H2O
-
 Vigna radiata alpha-D-glucose + phenyl alpha-D-glucoside
-
 ?
3.2.1.20 starch + H2O soluble Vigna radiata alpha-D-glucose
-
 ?
3.2.1.20 sucrose + H2O no activity Vigna radiata D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 105000, alpha-glucosidase II-1, SDS-PAGE Vigna radiata
3.2.1.20 ? x * 145000, alpha-glucosidase I, SDS-PAGE Vigna radiata
3.2.1.20 ? x * 65000, alpha-glucosidase II-2, SDS-PAGE Vigna radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 60
-
 alpha-glucosidase I Vigna radiata
3.2.1.20 65
-
 alpha-glucosidase II-1 and II-2 Vigna radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 65
-
 15 min, stable up to Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5
-
-
 Vigna radiata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3.5 6.5 30°C, 20 h, stable Vigna radiata