EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | Cobalamin | aquocobalamin | Methanothermobacter thermautotrophicus | |
2.8.4.1 | dithiothreitol | - |
Methanothermobacter thermautotrophicus | |
2.8.4.1 | additional information | maximal stimulation with 0.3 mM aquocobalamin and 0.15 mM Ti(III) citrate | Methanothermobacter thermautotrophicus | |
2.8.4.1 | reduced corrinoids | - |
Methanothermobacter thermautotrophicus | |
2.8.4.1 | Ti(III) citrate | - |
Methanothermobacter thermautotrophicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | 2-bromoethanesulfonate | competitive with methyl coenzyme M | Methanothermobacter thermautotrophicus | |
2.8.4.1 | CoM-S-S-CoB | 50% inhibition at 0.6 mM | Methanothermobacter thermautotrophicus | |
2.8.4.1 | N-6-mercaptohexanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus | |
2.8.4.1 | N-7(methylthio)-heptanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus | |
2.8.4.1 | N-8-mercaptooctanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.4.1 | 4 | - |
methyl coenzyme M | - |
Methanothermobacter thermautotrophicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.8.4.1 | cytoplasm | soluble | Methanothermobacter thermautotrophicus | 5737 | - |
2.8.4.1 | membrane | on the inner surface of the cytoplasmic membrane | Methanothermobacter thermautotrophicus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | Ni(2+) | - |
Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | additional information | - |
operon contains two additional polypeptides of 15752 and 21210 Da with unknown functions | Methanothermobacter thermautotrophicus |
2.8.4.1 | 37000 | - |
2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
2.8.4.1 | 48000 | - |
2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
2.8.4.1 | 66000 | - |
2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
2.8.4.1 | 150000 | - |
PAGE | Methanothermobacter thermautotrophicus |
2.8.4.1 | 300000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.4.1 | methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus | final step in methane formation in all methanogenic archaea | methane + CoM-S-S-CoB | - |
? | |
2.8.4.1 | methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | final step in methane formation in all methanogenic archaea | methane + CoM-S-S-CoB | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.4.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
2.8.4.1 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.4.1 | membrane filtration, on Mono Q, Superose-6 and Phenyl-Superose column | Methanothermobacter thermautotrophicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 0.075 | - |
up tp | Methanothermobacter thermautotrophicus |
2.8.4.1 | 0.5 | 2 | in presence of dithiothreitol and of reduced corrinoids or Ti(III) citrate | Methanothermobacter thermautotrophicus |
2.8.4.1 | 1 | 2 | in presence of 15 mM dithiothreitol and 0.3 mM aquocobalamin or 0.15 mM Ti(III) citrate | Methanothermobacter thermautotrophicus |
2.8.4.1 | 3 | 5 | cell extract | Methanothermobacter thermautotrophicus |
2.8.4.1 | 3 | 5 | drops to 1% after cell breakage | Methanothermobacter thermautotrophicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.4.1 | 4°C, under N2 atmosphere, 50 mM potassium phosphate, pH 6.7, 50% decrease of specific acitivity within 12 hours | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.4.1 | methyl coenzyme M + coenzyme B | final step in methane formation in all methanogenic archaea | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
2.8.4.1 | methyl coenzyme M + coenzyme B | final step in methane formation in all methanogenic archaea | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methane + CoM-S-S-CoB | - |
? | |
2.8.4.1 | methyl-coenzyme M + coenzyme B | specific for L-enantiomer of coenzyme B | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
2.8.4.1 | methyl-coenzyme M + coenzyme B | specific for L-enantiomer of coenzyme B | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methane + CoM-S-S-CoB | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.4.1 | hexamer | alpha2,beta2,gamma2 | Methanothermobacter thermautotrophicus |
2.8.4.1 | hexamer | 2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE | Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 65 | - |
assay at | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 6.7 | - |
assay at | Methanothermobacter thermautotrophicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | F-430 | 1.6-1.8 mol F-430/mol enzyme | Methanothermobacter thermautotrophicus | |
2.8.4.1 | F-430 | nickel porphinoid coenzyme M | Methanothermobacter thermautotrophicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.4.1 | 0.0001 | - |
N-6-mercaptohexanoylthreonine phosphate | competitive with coenzyme B | Methanothermobacter thermautotrophicus |