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Literature summary extracted from

  • Rospert, S.; Boecher, R.; Albracht, S.P.J.; Thauer, R.K.
    Methyl-coenzyme M reductase preparations with high specific activity from hydrogen-preincubated cells of Methanobacterium thermoautotrophicum (1991), FEBS Lett., 291, 371-375.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.4.1 methyl-coenzyme M stabilizes enzyme activity Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Ni(2+) involved in catalytic mechanism Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 300000
-
-
 Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus energy metabolism of all methanogenic archaea methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 energy metabolism of all methanogenic archaea methane + CoM-S-S-CoB
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter thermautotrophicus
-
-
-
2.8.4.1 Methanothermobacter thermautotrophicus
-
 cells preincubated with 100% H2 before desintegration, prduces two isoenzymes
-
2.8.4.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 all buffers that are used during purification process are supplemented with stabilising methyl coenzyme M, further chromatography steps with phenyl-superose, Mono-Q and Sephadex G25 result in 90% loss of specific activity Methanothermobacter thermautotrophicus
2.8.4.1 under 5% H2/95% N2 condition, fractionated ammonium sulfate precipitation Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.4.1 additional information
-
 enzyme shows only little activity in ox1 state, highly active in red1 or red2 form Methanothermobacter thermautotrophicus
2.8.4.1 0.72
-
 CO2-oxidized cells Methanothermobacter thermautotrophicus
2.8.4.1 20.1
-
 purification from H2-reduced cells Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
2.8.4.1 0°C, enzyme preparation, 100% H2 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B energy metabolism of all methanogenic archaea Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B energy metabolism of all methanogenic archaea Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 60
-
 assay Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 7
-
 assay at Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 2 mol coenzyme F-430/mol enzyme Methanothermobacter thermautotrophicus
2.8.4.1 F-430 2 mol nickel porphinoid/mol enzyme, prosthetic group Methanothermobacter thermautotrophicus