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Literature summary extracted from

  • Ouzzine, M.; Gulberti, S.; Netter, P.; Magdalou, J.; Fournel-Gigleux, S.
    Structure/function of the human Ga1beta1,3-glucuronosyltransferase. Dimerization and functional activity are mediated by two crucial cysteine residues (2000), J. Biol. Chem., 275, 28254-28260.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.135 expression in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.135 C301A mutant is not N-glycosylated, molecular weight is about 4000 Da less than that of the wild-type protein, enzyme is completely inactive Homo sapiens
2.4.1.135 C33A mutation abolishes the ability of the protein to form dimers Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.135 N-Phenylmaleimide
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.135 additional information
-
additional information
-
Homo sapiens
2.4.1.135 0.09489
-
UDPglucuronate wild-type enzyme Homo sapiens
2.4.1.135 0.287
-
UDPglucuronate mutant enzyme C33A Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.135 43000
-
2 * 43000, SDS-PAGE after disulfide reduction Homo sapiens
2.4.1.135 85000
-
SDS-PAGE under nonreducing conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.135 additional information Homo sapiens expressed in Pichia pastoris ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.135 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.135 glycoprotein
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.135 additional information expressed in Pichia pastoris Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.135 dimer 2 * 43000, SDS-PAGE after disulfide reduction Homo sapiens